Literature summary for 5.3.1.1 extracted from

Chu, C.; Lai, Y.; Huang, H.; Sun, Y.
Kinetic and structural properties of triosephosphate isomerase from Helicobacter pylori (2008), Proteins Struct. Funct. Bioinform., 71, 396-406.

Search for more literature for 5.3.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
-	Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment)	Organism
monomer, to 2.3 A resolution	Helicobacter pylori

Protein Variants

Protein Variants	Comment	Organism
D213A	disruption of the salt bridge between residues D213 and K183, kinetic parameters similar to wild-type	Helicobacter pylori
D213Q	disruption of the salt bridge between residues D213 and K183, kinetic parameters similar to wild-type	Helicobacter pylori
K183A	disruption of the salt bridge between residues D213 and K183, kinetic parameters similar to wild-type	Helicobacter pylori
K183S	disruption of the salt bridge between residues D213 and K183, kinetic parameters similar to wild-type	Helicobacter pylori

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.49	-	D-glyceraldehyde 3-phosphate	mutant D213A, pH 7.5, 25°C	Helicobacter pylori
3.27	-	D-glyceraldehyde 3-phosphate	mutant D213Q, pH 7.5, 25°C	Helicobacter pylori
3.46	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.5, 25°C	Helicobacter pylori
3.56	-	D-glyceraldehyde 3-phosphate	mutant K183S, pH 7.5, 25°C	Helicobacter pylori
9.09	-	D-glyceraldehyde 3-phosphate	mutant K183A, pH 7.5, 25°C	Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28108	-	2 * 28108, mass spectrometry	Helicobacter pylori
56000	-	gel filtration	Helicobacter pylori

Organism

Organism	UniProt	Comment	Textmining
Helicobacter pylori	P56076	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Helicobacter pylori	dihydroxyacetone phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 28108, mass spectrometry	Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
14670	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.5, 25°C	Helicobacter pylori
15670	-	D-glyceraldehyde 3-phosphate	mutant D213A, pH 7.5, 25°C	Helicobacter pylori
18330	-	D-glyceraldehyde 3-phosphate	mutant D213Q, pH 7.5, 25°C	Helicobacter pylori
20000	-	D-glyceraldehyde 3-phosphate	mutant K183S, pH 7.5, 25°C	Helicobacter pylori
23330	-	D-glyceraldehyde 3-phosphate	mutant K183A, pH 7.5, 25°C	Helicobacter pylori

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)