Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli AA200 cells | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme C126S in complex with PGA, hanging drop vapor diffusion method, using 20% (w/v) poly(ethylene glycol), 1 M HEPES buffer (pH 7.5), and 10 mM lithium sulfate, at 23°C. Unliganded mutant enzymes C126S and C126A, hanging drop vapor diffusion method, using 24% (w/v) poly(ethylene glycol), 1 M HEPES buffer (pH 7.0), and 10 mM lithium sulfate | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
C126A | the mutant shows an approximately 5.8fold drop in kcat compared to the wild type enzyme | Plasmodium falciparum |
C126M | the mutant shows an approximately 10fold drop in catalytic activity compared to the wild type enzyme | Plasmodium falciparum |
C126S | the mutant shows an approximately 5.8fold drop in kcat compared to the wild type enzyme | Plasmodium falciparum |
C126T | the mutant shows an approximately 10fold drop in catalytic activity compared to the wild type enzyme | Plasmodium falciparum |
C126V | the mutant shows an approximately 10fold drop in catalytic activity compared to the wild type enzyme | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
1 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126V, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
1.2 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126T, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
1.4 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126S, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
1.5 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126A, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
1.5 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126M, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q07412 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl-200 gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Plasmodium falciparum | dihydroxyacetone phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Plasmodium falciparum |
Triosephosphate isomerase | - |
Plasmodium falciparum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | for the wild type enzyme, there is an increase in activity over the temperature range 25-40°C, with a leveling off between 40°C and 50°C | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126V, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
190 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126M, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
330 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126T, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
750 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126S, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
770 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126A, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
4300 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
120 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126M, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
160 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126V, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
280 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126T, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
520 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126A, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
540 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C126S, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum | |
12000 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C | Plasmodium falciparum |