Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.1 extracted from

  • Aguirre, B.; Costas, M.; Cabrera, N.; Mendoza-Hernandez, G.; Helseth, D.L.; Fernandez, P.; Tuena de Gomez-Puyou, M.; Perez-Montfort, R.; Torres-Larios, A.; Gomez Puyou, A.
    A Ribosomal misincorporation of Lys for Arg in human triosephosphate isomerase expressed in Escherichia coli gives rise to two protein populations (2011), PLoS ONE, 6, e21035.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) or BL21-CodonPlus(DE3)RIL cells. The preparations of native TIM contain two isolatable stable catalytically active forms P1 and P2 of TIM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
D-glyceraldehyde 3-phosphate active form P2 of TIM, at 25°C, pH 7.4 Homo sapiens
0.3
-
D-glyceraldehyde 3-phosphate active form P1 of TIM, at 25°C, pH 7.4 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26732
-
2 * 26732, calculated from amino acid sequence Homo sapiens
54000
-
gel filtration Homo sapiens

Organic Solvent Stability

Organic Solvent Comment Organism
urea the enzyme is denatured in buffer with 3 M urea Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glyceraldehyde 3-phosphate
-
Homo sapiens Glycerone phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 26732, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
TIM
-
Homo sapiens
Triosephosphate isomerase
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
the melting temperature of the active form P1 of TIM is 48°C Homo sapiens
64
-
the melting temperature of the active form P2 of TIM is 64°C Homo sapiens