Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) or BL21-CodonPlus(DE3)RIL cells. The preparations of native TIM contain two isolatable stable catalytically active forms P1 and P2 of TIM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
D-glyceraldehyde 3-phosphate | active form P2 of TIM, at 25°C, pH 7.4 | Homo sapiens | |
0.3 | - |
D-glyceraldehyde 3-phosphate | active form P1 of TIM, at 25°C, pH 7.4 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26732 | - |
2 * 26732, calculated from amino acid sequence | Homo sapiens |
54000 | - |
gel filtration | Homo sapiens |
Organic Solvent | Comment | Organism |
---|---|---|
urea | the enzyme is denatured in buffer with 3 M urea | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Homo sapiens | Glycerone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 26732, calculated from amino acid sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Homo sapiens |
Triosephosphate isomerase | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
the melting temperature of the active form P1 of TIM is 48°C | Homo sapiens |
64 | - |
the melting temperature of the active form P2 of TIM is 64°C | Homo sapiens |