Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli AA200 cells (a null mutant of inherent TIM gene) | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
C13D | the mutant displays significant reduction in catalytic activity when compared with wild type enzyme (about 7.4fold decrease in kcat). The C13D mutant dissociates at concentrations above 1.25 mM | Plasmodium falciparum |
C13E | the mutant displays significant reduction in catalytic activity when compared with wild type enzyme (about 3.3fold decrease in kcat). The C13E mutant retains dimeric at concentrations above 1.25 mM | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C13D, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication | Plasmodium falciparum | |
0.35 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication | Plasmodium falciparum | |
0.42 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C13E, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q07412 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl-200 gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Plasmodium falciparum | dihydroxyacetone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Plasmodium falciparum |
Triosephosphate isomerase | - |
Plasmodium falciparum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the melting temperature of the wild type enzyme is at 60°C | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
600 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C13D, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication | Plasmodium falciparum | |
1367 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C13E, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication | Plasmodium falciparum | |
4467 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1933 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C13D, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication | Plasmodium falciparum | |
3250 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme C13E, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication | Plasmodium falciparum | |
12750 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication | Plasmodium falciparum |