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Literature summary for 5.3.1.1 extracted from

  • Kumar, K.; Bhargava, P.; Roy, U.
    Cloning, overexpression and characterization of Leishmania donovani triosephosphate isomerase (2012), Exp. Parasitol., 130, 430-436.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
methylmethane thiosulfonate induces 75% enzyme inactivation within 15 min at 0.25 mM concentration Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.328
-
D-glyceraldehyde 3-phosphate in 50 mM Tris, pH 7.6, at 25°C Leishmania donovani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27600
-
2 * 27600, estimated from SDS-PAGE Leishmania donovani
54000
-
gel filtration Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate Leishmania donovani
-
dihydroxyacetone phosphate
-
?
D-glyceraldehyde 3-phosphate Leishmania donovani MHOM/IN/80/Dd8
-
dihydroxyacetone phosphate
-
?

Organism

Organism UniProt Comment Textmining
Leishmania donovani
-
-
-
Leishmania donovani MHOM/IN/80/Dd8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Q-Sepharose column chromatography and Sephadex G-100 gel filtration Leishmania donovani

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.01
-
total cell lysate, in 50 mM Tris, pH 7.6, at 25°C Leishmania donovani
8.76
-
after 8.7fold purification, in 50 mM Tris, pH 7.6, at 25°C Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate
-
Leishmania donovani dihydroxyacetone phosphate
-
?
D-glyceraldehyde 3-phosphate
-
Leishmania donovani MHOM/IN/80/Dd8 dihydroxyacetone phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 27600, estimated from SDS-PAGE Leishmania donovani

Synonyms

Synonyms Comment Organism
TIM
-
Leishmania donovani
Triosephosphate isomerase
-
Leishmania donovani

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 60 the recombinant enzyme is stable at 25°C for 30 min. The recombinant enzyme retains 92.7%, 40% and 13% activity when preincubated at 37°C, 42°C and 60°C for 30 min, respectively Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 9
-
Leishmania donovani

pH Range

pH Minimum pH Maximum Comment Organism
7 10 the recombinant protein shows more than 90% activity over the pH range 7.2-9.0 and less than 70% activity below pH 7.0 or pH above pH 10.0 Leishmania donovani