Literature summary for 5.3.1.1 extracted from

Jimenez-Sandoval, P.; Vique-Sanchez, J.L.; Hidalgo, M.L.; Velazquez-Juarez, G.; Diaz-Quezada, C.; Arroyo-Navarro, L.F.; Moran, G.M.; Fattori, J.; Jessica Diaz-Salazar, A.; Rudino-Pinera, E.; Sotelo-Mundo, R.; Figueira, A.C.M.; Lara-Gonzalez, S.; Benitez-Cardoza, C.G.; Brieba, L.G.
A competent catalytic active site is necessary for substrate induced dimer assembly in triosephosphate isomerase (2017), Biochim. Biophys. Acta, 1865, 1423-1432 .

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Protein Variants

Protein Variants	Comment	Organism
I45A	39fold less active than wild-type. Mutant dissociates into stable monomers and assembles as catalytic competent dimer upon binding of the transition state substrate analog phosphoglycolohydroxamate	Trichomonas vaginalis
additional information	a mutant lacking loop 3 is 1500fold less active than wild-type and dissociates into stable monomers. Upon binding of the transition state substrate analog phosphoglycolohydroxamate, mutant remains monomeric	Trichomonas vaginalis
additional information	a mutant lacking loop 3 is 9400fold less active than wild-type and dissociates into stable monomers. Upon binding of the transition state substrate analog phosphoglycolohydroxamate, mutant remains monomeric	Trichomonas vaginalis
V45A	29fold less active than wild-type. Mutant dissociates into stable monomers and assembles as catalytic competent dimer upon binding of the transition state substrate analog phosphoglycolohydroxamate	Trichomonas vaginalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.21	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.4, 25°C	Trichomonas vaginalis
0.23	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.4, 25°C	Trichomonas vaginalis
0.39	-	D-glyceraldehyde 3-phosphate	mutant lacking loop 3, pH 7.4, 25°C	Trichomonas vaginalis
0.5	-	D-glyceraldehyde 3-phosphate	mutant lacking loop 3, pH 7.4, 25°C	Trichomonas vaginalis
0.51	-	D-glyceraldehyde 3-phosphate	mutant V45A, pH 7.4, 25°C	Trichomonas vaginalis

Organism

Organism	UniProt	Comment	Textmining
Trichomonas vaginalis	A2EGX9	-	-
Trichomonas vaginalis	A2FT29	isoform Tim2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Trichomonas vaginalis	dihydroxyacetone 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
TIM2	-	Trichomonas vaginalis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.31	-	D-glyceraldehyde 3-phosphate	mutant lacking loop 3, pH 7.4, 25°C	Trichomonas vaginalis
1.5	-	D-glyceraldehyde 3-phosphate	mutant lacking loop 3, pH 7.4, 25°C	Trichomonas vaginalis
76.7	-	D-glyceraldehyde 3-phosphate	mutant V45A, pH 7.4, 25°C	Trichomonas vaginalis
1330	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.4, 25°C	Trichomonas vaginalis
1373	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.4, 25°C	Trichomonas vaginalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.62	-	D-glyceraldehyde 3-phosphate	mutant lacking loop 3, pH 7.4, 25°C	Trichomonas vaginalis
3.9	-	D-glyceraldehyde 3-phosphate	mutant lacking loop 3, pH 7.4, 25°C	Trichomonas vaginalis
149	-	D-glyceraldehyde 3-phosphate	mutant V45A, pH 7.4, 25°C	Trichomonas vaginalis
5833	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.4, 25°C	Trichomonas vaginalis
6333	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.4, 25°C	Trichomonas vaginalis

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Release 2023.1 (January 2023)