Literature summary for 5.3.1.1 extracted from

Lara-Gonzalez, S.; Estrella, P.; Portillo, C.; Cruces, M.E.; Jimenez-Sandoval, P.; Fattori, J.; Migliorini-Figueira, A.C.; Lopez-Hidalgo, M.; Diaz-Quezada, C.; Lopez-Castillo, M.; Trasvina-Arenas, C.H.; Sanchez-Sandoval, E.; Gomez-Puyou, A.; Ortega-Lopez, J.; Arroyo, R.; Benitez-Cardoza, C.G.; Brieba, L.
Substrate-induced dimerization of engineered monomeric variants of triosephosphate isomerase from Trichomonas vaginalis (2015), PLoS ONE, 10, e0141747 .

Protein Variants

Protein Variants	Comment	Organism
I45A	29fold decrease in activity. Residue I45 controls the dimer-monomer equilibrium, mutant displays a dimer-monomer equilibrium and is stable	Trichomonas vaginalis
I45F	dimer-monomer equilibrium	Trichomonas vaginalis
I45G	142fold decrease in catalytic activity	Trichomonas vaginalis
I45L	dimeric, CD spectrum is identical to wild-type	Trichomonas vaginalis
I45V	dimeric, CD spectrum is identical to wild-type	Trichomonas vaginalis
I45W	insoluble	Trichomonas vaginalis
I45Y	monomeric, 479fold decrease in catalytic activity	Trichomonas vaginalis

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.23	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.4, 25°C	Trichomonas vaginalis
0.3	-	D-glyceraldehyde 3-phosphate	mutant I45V, pH 7.4, 25°C	Trichomonas vaginalis
0.32	-	D-glyceraldehyde 3-phosphate	mutant I45L, pH 7.4, 25°C	Trichomonas vaginalis
0.39	-	D-glyceraldehyde 3-phosphate	mutant I45A, pH 7.4, 25°C	Trichomonas vaginalis
0.52	-	D-glyceraldehyde 3-phosphate	mutant I45F, pH 7.4, 25°C	Trichomonas vaginalis
1.29	-	D-glyceraldehyde 3-phosphate	mutant I45Y, pH 7.4, 25°C	Trichomonas vaginalis
1.78	-	D-glyceraldehyde 3-phosphate	mutant I45G, pH 7.4, 25°C	Trichomonas vaginalis

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	gel filtration	Trichomonas vaginalis

Organism	UniProt	Comment	Textmining
Trichomonas vaginalis	A2EGX9	-	-

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Trichomonas vaginalis	dihydroxyacetone 3-phosphate	-	?

Subunits	Comment	Organism
dimer	2 * 27000, calculated from sequence	Trichomonas vaginalis

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	dimeric enzyme unfolds following a four state model denaturation process whereas monomeric variants follow a three state model	Trichomonas vaginalis

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
15.7	-	D-glyceraldehyde 3-phosphate	mutant I45Y, pH 7.4, 25°C	Trichomonas vaginalis
17.7	-	D-glyceraldehyde 3-phosphate	mutant I45F, pH 7.4, 25°C	Trichomonas vaginalis
72.8	-	D-glyceraldehyde 3-phosphate	mutant I45G, pH 7.4, 25°C	Trichomonas vaginalis
78.3	-	D-glyceraldehyde 3-phosphate	mutant I45A, pH 7.4, 25°C	Trichomonas vaginalis
1330	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.4, 25°C	Trichomonas vaginalis
1650	-	D-glyceraldehyde 3-phosphate	mutant I45V, pH 7.4, 25°C	Trichomonas vaginalis
1900	-	D-glyceraldehyde 3-phosphate	mutant I45L, pH 7.4, 25°C	Trichomonas vaginalis

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
12.2	-	D-glyceraldehyde 3-phosphate	mutant I45Y, pH 7.4, 25°C	Trichomonas vaginalis
33.3	-	D-glyceraldehyde 3-phosphate	mutant I45F, pH 7.4, 25°C	Trichomonas vaginalis
40.8	-	D-glyceraldehyde 3-phosphate	mutant I45G, pH 7.4, 25°C	Trichomonas vaginalis
198.3	-	D-glyceraldehyde 3-phosphate	mutant I45A, pH 7.4, 25°C	Trichomonas vaginalis
5467	-	D-glyceraldehyde 3-phosphate	mutant I45V, pH 7.4, 25°C	Trichomonas vaginalis
5833	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.4, 25°C	Trichomonas vaginalis
5867	-	D-glyceraldehyde 3-phosphate	mutant I45L, pH 7.4, 25°C	Trichomonas vaginalis