Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21(DE3)pLysS | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.25 mM, 50% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
D-glucuronate | in absence of added metal or chelator | Escherichia coli | |
0.31 | - |
D-glucuronate | in presence of 0.01 mM Zn2+ | Escherichia coli | |
0.41 | - |
D-glucuronate | in presence of 0.01 mM Co2+ | Escherichia coli | |
1.2 | - |
D-glucuronate | in presence of 0.1 mM Mn2+ | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | stimulation is more potent at lower concentrations than stimulation by Mn2+ | Escherichia coli | |
Mn2+ | maximal stimulation is higher than stimulatipn by Zn2+ or Co2+, stimulation at low concentrations is lower than stimulation by Zn2+ and Co2+ | Escherichia coli | |
Zn2+ | stimulation is more potent at lower concentrations than stimulation by Mn2+. Vmax is about 3times higher in presence of 0.1 mM Mn2+ than in presence of 0.01 mM Zn2+ or Co2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucuronate | - |
Escherichia coli | D-Fructuronate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Uronate isomerase | - |
Escherichia coli |