Literature summary for 5.3.1.12 extracted from

Nguyen, T.T.; Brown, S.; Fedorov, A.A.; Fedorov, E.V.; Babbitt, P.C.; Almo, S.C.; Raushel, F.M.
At the periphery of the amidohydrolase superfamily: Bh0493 from Bacillus halodurans catalyzes the isomerization of D-galacturonate to D-tagaturonate (2008), Biochemistry, 47, 1194-1206.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Halalkalibacterium halodurans

Crystallization (Commentary)

Crystallization (Comment)	Organism
complexed with Zn2+, vapour diffusion method, with 45% polypropylene glycol and 0.1 M Bis-Tris, pH 6.5	Halalkalibacterium halodurans

Inhibitors

Inhibitors	Comment	Organism	Structure
(2S,3R,4S)-4-(hydroxycarbamoyl)-2,3,4-trihydroxybutanoate	-	Halalkalibacterium halodurans
D-arabinaric acid	-	Halalkalibacterium halodurans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	D-glucuronate	at 30°C, pH 8.0	Halalkalibacterium halodurans
0.05	-	D-galacturonate	at 30°C, pH 8.0	Halalkalibacterium halodurans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	contains 0.5 equivalents of Zn2+ per subunit	Halalkalibacterium halodurans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49000	-	SDS-PAGE	Halalkalibacterium halodurans

Organism

Organism	UniProt	Comment	Textmining
Halalkalibacterium halodurans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Resource Q column chromatography and Superdex 200 gel filtration	Halalkalibacterium halodurans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Galacturonate	-	Halalkalibacterium halodurans	D-Tagaturonate	-	?
D-Glucuronate	-	Halalkalibacterium halodurans	D-Fructuronate	-	?

Subunits

Subunits	Comment	Organism
trimer	x-ray crystallography	Halalkalibacterium halodurans

Synonyms

Synonyms	Comment	Organism
Bh0493	-	Halalkalibacterium halodurans
Uronate isomerase	-	Halalkalibacterium halodurans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.4	-	D-galacturonate	at 30°C, pH 8.0	Halalkalibacterium halodurans
5.2	-	D-glucuronate	at 30°C, pH 8.0	Halalkalibacterium halodurans

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0021	-	(2S,3R,4S)-4-(hydroxycarbamoyl)-2,3,4-trihydroxybutanoate	at 30°C, pH 8.0	Halalkalibacterium halodurans
0.0055	-	D-arabinaric acid	at 30°C, pH 8.0	Halalkalibacterium halodurans

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Release 2023.1 (January 2023)