Literature summary for 5.3.1.14 extracted from

Leang, K.; Takada, G.; Fukai, Y.; Morimoto, K.; Granstrom, T.B.; Izumori, K.
Novel reactions of L-rhamnose isomerase from Pseudomonas stutzeri and its relation with D-xylose isomerase via substrate specificity (2004), Biochim. Biophys. Acta, 1674, 68-77.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Pseudomonas stutzeri

Inhibitors	Comment	Organism
Ag+	1 mM, complete inhibition	Pseudomonas stutzeri
Ba2+	1 mM, 97% inhibition	Pseudomonas stutzeri
Ca2+	1 mM, 88% inhibition	Pseudomonas stutzeri
Co2+	1 mM, 79% inhibition	Pseudomonas stutzeri
Cu2+	1 mM, 61% inhibition	Pseudomonas stutzeri
EDTA	1 mM, 98% inhibition	Pseudomonas stutzeri
Fe2+	1 mM, complete inhibition	Pseudomonas stutzeri
Mg2+	1 mM, 45% inhibition	Pseudomonas stutzeri
Na+	1 mM, 12% inhibition	Pseudomonas stutzeri
Ni2+	1 mM, 97% inhibition	Pseudomonas stutzeri
Zn2+	1 mM, 89% inhibition	Pseudomonas stutzeri

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
11	-	L-rhamnulose	-	Pseudomonas stutzeri
38	-	D-ribose	-	Pseudomonas stutzeri
42	-	D-allose	-	Pseudomonas stutzeri
54	-	L-Mannose	-	Pseudomonas stutzeri
61	-	L-Lyxose	-	Pseudomonas stutzeri
71	-	D-altrose	-	Pseudomonas stutzeri
127	-	D-arabinose	-	Pseudomonas stutzeri
202	-	L-Xylose	-	Pseudomonas stutzeri
250	-	D-xylose	-	Pseudomonas stutzeri
564	-	D-glucose	-	Pseudomonas stutzeri

Metals/Ions	Comment	Organism
Al3+	1 mM, activates to 104% of control	Pseudomonas stutzeri
Li+	1 mM, activates to 106% of control	Pseudomonas stutzeri
Mn2+	1 mM, activates to 112% of control	Pseudomonas stutzeri

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	-	x * 43000, recombinant His-tagged enzyme, SDS-PAGE	Pseudomonas stutzeri

Organism	UniProt	Comment	Textmining
Pseudomonas stutzeri	-	-	-

Purification (Comment)	Organism
recombinant His-tagged enzyme	Pseudomonas stutzeri

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Pseudomonas stutzeri

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-allose	-	Pseudomonas stutzeri	D-psicose + D-altrose	-	?
D-altrose	-	Pseudomonas stutzeri	D-psicose + D-altrose	-	r
D-arabinose	-	Pseudomonas stutzeri	D-ribulose + D-ribose	-	r
D-erythrose	-	Pseudomonas stutzeri	D-erythrulose + D-threose	-	r
D-galactose	-	Pseudomonas stutzeri	D-tagatose	-	r
D-glucose	-	Pseudomonas stutzeri	D-fructose + D-mannose	-	r
D-gulose	-	Pseudomonas stutzeri	D-sorbose	-	r
D-lyxose	-	Pseudomonas stutzeri	D-xylulose + D-xylose	-	r
D-ribose	-	Pseudomonas stutzeri	D-ribulose + D-arabinose	-	r
D-threose	-	Pseudomonas stutzeri	D-erythrulose + D-erythrose	-	r
D-xylose	-	Pseudomonas stutzeri	D-xylulose + D-lyxose	-	r
L-arabinose	-	Pseudomonas stutzeri	L-ribulose	-	r
L-erythrose	-	Pseudomonas stutzeri	L-erythrulose + L-threose	-	r
L-galactose	-	Pseudomonas stutzeri	L-tagatose + L-talose	-	r
L-glucose	-	Pseudomonas stutzeri	L-fructose + L-mannose	-	r
L-lyxose	-	Pseudomonas stutzeri	L-xylulose + L-xylose	-	r
L-mannose	-	Pseudomonas stutzeri	L-fructose + L-glucose	-	r
L-rhamnulose	-	Pseudomonas stutzeri	L-rhamnulose	-	r
L-ribose	-	Pseudomonas stutzeri	L-ribulose + L-arabinose	-	r
L-threose	-	Pseudomonas stutzeri	L-erythrulose + L-erythrose	-	r
L-xylose	-	Pseudomonas stutzeri	L-xylulose + L-lyxose	-	r

Subunits	Comment	Organism
?	x * 43000, recombinant His-tagged enzyme, SDS-PAGE	Pseudomonas stutzeri

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.017	-	L-Xylose	-	Pseudomonas stutzeri
0.024	-	D-glucose	-	Pseudomonas stutzeri
0.05	-	D-altrose	-	Pseudomonas stutzeri
0.17	-	D-arabinose	-	Pseudomonas stutzeri
33	-	D-xylose	-	Pseudomonas stutzeri
900	-	L-Lyxose	-	Pseudomonas stutzeri
1000	-	L-Mannose	-	Pseudomonas stutzeri
1717	-	L-rhamnulose	-	Pseudomonas stutzeri
2500	-	D-allose	-	Pseudomonas stutzeri
3150	-	D-ribose	-	Pseudomonas stutzeri