Literature summary for 5.3.1.15 extracted from

Cho, E.A.; Lee, D.W.; Cha, Y.H.; Lee, S.J.; Jung, H.C.; Pan, J.G.; Pyun, Y.R.
Characterization of a novel D-lyxose isomerase from Cohnella laevoribosii RI-39 sp. nov (2007), J. Bacteriol., 189, 1655-1663.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Cohnella laeviribosi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
22.4	-	D-Lyxose	-	Cohnella laeviribosi
34	-	D-mannose	-	Cohnella laeviribosi
121.7	-	L-ribose	-	Cohnella laeviribosi

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	activating at 1 mM, D-xylose as substrate	Cohnella laeviribosi
Mn2+	at 1 mM, D-xylose as substrate, requires divalent metal ions	Cohnella laeviribosi
Ni2+	activating at 1 mM, D-xylose as substrate	Cohnella laeviribosi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42000	-	gel filtration	Cohnella laeviribosi

Organism

Organism	UniProt	Comment	Textmining
Cohnella laeviribosi	A3E7Z6	-	-
Cohnella laeviribosi RI-39	A3E7Z6	-	-

Purification (Commentary)

Purification (Comment)	Organism
of the native protein to homogeneity by DEAE-Sephacel ion-exchange chromatography, followed by RESOURCE PHE hydrophobic chromatography, of the recombinant protein	Cohnella laeviribosi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
8.4	-	of the native enzyme after purification, at 70°C, with L-ribose as substrate	Cohnella laeviribosi
50.2	-	of the recombinant enzyme after purification, at 70°C, with L-ribose as substrate	Cohnella laeviribosi

Storage Stability

Storage Stability	Organism
at -20°C for several months	Cohnella laeviribosi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Lyxose	-	Cohnella laeviribosi	D-Xylulose	-	r
D-Lyxose	-	Cohnella laeviribosi RI-39	D-Xylulose	-	r
D-Mannose	no activity with L-lyxose, Dxylose,L-xylose, D-arabinose, L-arabinose, D-ribose, L-fucose, D-glucose, D-galactose, D-erythrose, L-rhamnose, 2-deoxy-D-ribose, and ribose-5-phosphate	Cohnella laeviribosi	D-Fructose	-	r
D-Mannose	no activity with L-lyxose, Dxylose,L-xylose, D-arabinose, L-arabinose, D-ribose, L-fucose, D-glucose, D-galactose, D-erythrose, L-rhamnose, 2-deoxy-D-ribose, and ribose-5-phosphate	Cohnella laeviribosi RI-39	D-Fructose	-	r
L-ribose	-	Cohnella laeviribosi	L-ribulose	-	r
L-ribose	-	Cohnella laeviribosi RI-39	L-ribulose	-	r

Subunits

Subunits	Comment	Organism
dimer	2 x 21000 SDS-PAGE	Cohnella laeviribosi

Synonyms

Synonyms	Comment	Organism
CLLI	D-lyxose isomerase from Cohnella laevoribosii	Cohnella laeviribosi
CLRI	L-ribose isomerase from Cohnella laevoribosii	Cohnella laeviribosi
D-Lyxose isomerase	-	Cohnella laeviribosi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	wild-type and recombinant enzymes at 1 mM Mn2+	Cohnella laeviribosi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	wild-type and recombinant enzymes at 1 mM Mn2+	Cohnella laeviribosi

pI Value

Organism	Comment	pI Value Maximum	pI Value
Cohnella laeviribosi	wild-type and recombinant enzymes	-	4.2

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Release 2023.1 (January 2023)