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Literature summary for 5.3.1.15 extracted from

  • Kwon, H.J.; Yeom, S.J.; Park, C.S.; Oh, D.K.
    Substrate specificity of a recombinant D-lyxose isomerase from Providencia stuartii for monosaccharides (2010), J. Biosci. Bioeng., 110, 26-31.
    View publication on PubMed
Search for more literature for 5.3.1.15

Application

Application Comment Organism
food industry the enzyme is an industrial producer of D-lyxose Providencia stuartii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Providencia stuartii

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ about 80% residual activity at 1 mM Providencia stuartii
Co2+ about 15% residual activity at 1 mM Providencia stuartii
Cu2+ about 55% residual activity at 1 mM Providencia stuartii
EDTA 12% residual activity at 1 mM Providencia stuartii
Fe2+ about 60% residual activity at 1 mM Providencia stuartii
additional information not affected by Ca2+ Providencia stuartii
Zn2+ about 60% residual activity at 1 mM Providencia stuartii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
22
-
 D-mannose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
38
-
 D-xylulose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
47
-
 D-Lyxose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
54
-
 D-fructose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ about 150% activity at 1 mM Mg2+ Providencia stuartii
Mn2+ maximal activity (about 250%) in the presence of 1 mM Mn2+ Providencia stuartii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 2 * 22000, SDS-PAGE Providencia stuartii
22160
-
 2 * 22160, calculated from amino acid sequence Providencia stuartii
44000
-
 gel filtration Providencia stuartii

Organism

Organism UniProt Comment Textmining
Providencia stuartii
-
-
-
Providencia stuartii KCTC 2568
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography, gel filtration Providencia stuartii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 using L-tagatose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.003
-
 using L-mannose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.005
-
 using D-psicose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.007
-
 using L-lyxose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.01
-
 using L-psicose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.011
-
 using L-talose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.013
-
 using D-allose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.014
-
 using D-ribose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.015
-
 using D-tagatose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.017
-
 using L-fructose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.022
-
 using L-allose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.045
-
 using D-tagatose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.096
-
 using D-ribulose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.18
-
 using L-xylulose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.219
-
 using L-ribose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.403
-
 using D-fructose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.523
-
 using D-mannose as substrate, at pH 7.5 and 45°C Providencia stuartii
0.872
-
 using L-ribulose as substrate, at pH 7.5 and 45°C Providencia stuartii
22.33
-
 using D-lyxose as substrate, at pH 7.5 and 45°C Providencia stuartii
24.04
-
 using D-xylulose as substrate, at pH 7.5 and 45°C Providencia stuartii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-allose
-
 Providencia stuartii D-psicose
-
 r
D-allose
-
 Providencia stuartii KCTC 2568 D-psicose
-
 r
D-fructose
-
 Providencia stuartii D-mannose
-
 r
D-Lyxose highest specific activity Providencia stuartii D-Xylulose
-
 r
D-Mannose
-
 Providencia stuartii D-Fructose
-
 r
D-ribose
-
 Providencia stuartii D-ribulose
-
 r
D-ribose
-
 Providencia stuartii KCTC 2568 D-ribulose
-
 r
D-talose
-
 Providencia stuartii D-tagatose
-
 r
D-xylulose
-
 Providencia stuartii D-lyxose
-
 r
L-allose
-
 Providencia stuartii L-psicose
-
 r
L-lyxose
-
 Providencia stuartii L-xylulose
-
 r
L-lyxose
-
 Providencia stuartii KCTC 2568 L-xylulose
-
 r
L-Mannose
-
 Providencia stuartii L-Fructose
-
 r
L-Mannose
-
 Providencia stuartii KCTC 2568 L-Fructose
-
 r
L-ribose
-
 Providencia stuartii L-ribulose
-
 r
L-talose
-
 Providencia stuartii L-tagatose
-
 r
additional information the enzyme does not isomerizes D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate Providencia stuartii ?
-
 ?
additional information the enzyme does not isomerizes D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate Providencia stuartii KCTC 2568 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 22000, SDS-PAGE Providencia stuartii
homodimer 2 * 22160, calculated from amino acid sequence Providencia stuartii

Synonyms

Synonyms Comment Organism
D-Lyxose isomerase
-
 Providencia stuartii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Providencia stuartii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50 at 40°C and 50°C, the activities are approximately 60% of the maximum Providencia stuartii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the enzyme shows half-lives of 36 h, 14 h, 8.9 h, 3.4 h, and 1.4 h at 30°C, 35°C, 40°C, 45°C, and 50 °C, respectively Providencia stuartii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2010
-
 D-fructose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
2640
-
 D-mannose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
31340
-
 D-Lyxose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
34960
-
 D-xylulose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Providencia stuartii

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 at pH 7.0 and 8.5, the activities are approximately 60% of the maximum Providencia stuartii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
37
-
 D-fructose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
116
-
 D-mannose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
669
-
 D-Lyxose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
920
-
 D-xylulose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii