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Literature summary for 5.3.1.20 extracted from
- Purification, crystallization, and properties of D-ribose isomerase from Mycobacterium smegmatis (1975), J. Biol. Chem., 250, 8085-8087.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Mycolicibacterium smegmatis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-arabitol | - |
Mycolicibacterium smegmatis |  |
| ribitol | - |
Mycolicibacterium smegmatis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
D-ribose | - |
Mycolicibacterium smegmatis | |
| 5.3 | - |
L-Lyxose | - |
Mycolicibacterium smegmatis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation required, Km: 0.0004 mM | Mycolicibacterium smegmatis | |
| Mg2+ | divalent cation required, Km: 0.018 mM | Mycolicibacterium smegmatis | |
| Mn2+ | divalent cation required for activity, Mn2+ is the best activator, Km: 0.0001 mM | Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 145000 | 174000 | sedimentation equilibrium analysis | Mycolicibacterium smegmatis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.84 | - |
- |
Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Allose | - |
Mycolicibacterium smegmatis | ? | - |
? | |
| D-Lyxose | - |
Mycolicibacterium smegmatis | ? | - |
? | |
| D-ribose | - |
Mycolicibacterium smegmatis | D-ribulose | - |
? | |
| L-lyxose | - |
Mycolicibacterium smegmatis | ? | - |
? | |
| L-Rhamnose | - |
Mycolicibacterium smegmatis | L-Rhamnulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 42000-44000, SDS-PAGE in presence of Mn2+ | Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Mycolicibacterium smegmatis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | pH 6.5: about 45% of maximal activity, pH 9.5: about 55% of maximal activity | Mycolicibacterium smegmatis |
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