Application | Comment | Organism |
---|---|---|
biotechnology | the work provides a method of exchanging variably sized loops within the (beta/alpha)8 fold, affording a novel starting point for the screening of novel activities as well as modest diversions from an original activity | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
the expression of PRAI-LoxP variants is performed in Escherichia coli Rosetta 2 cells transformed with pET28b+ plasmids containing the encoding sequences | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Mut_L2_FBPA | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 2, sequence of loop SNGGASFIAGKGVKSDVPQ, fructose-bisphosphate aldolase, EC 4.1.2.13 | Escherichia coli |
Mut_L2_MR | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 2, sequence of loop GYPAL, mandelate racemase, EC 5.1.2.2 | Escherichia coli |
Mut_L2_PRAI_WT | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 2, sequence of loop VATSPRCVN, phosphoribosylanthranilate isomerase, EC 5.3.1.24 | Escherichia coli |
Mut_L2_Ure | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 2, sequence of loop GGTGPAAGTHATTCTPG, urease, EC 3.5.1.5 | Escherichia coli |
Mut_L4_ADA | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop GDELGFPGSLF, adenosine diaminase, EC 3.5.4.4 | Escherichia coli |
Mut_L4_alphaTS | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop DVPVQQS, tryptophan synthase, EC 4.2.1.20 | Escherichia coli |
Mut_L4_DHDPS | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop PYYNRPS, dihydrodipicolinate synthase, EC 4.2.1.52 | Escherichia coli |
Mut_L4_FBPA | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop DLSEES, fructose-bisphosphate aldolase, EC 4.1.2.13 | Escherichia coli |
Mut_L4_MR | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop EPTLEHD, mandelate racemase, EC 5.1.2.2 | Escherichia coli |
Mut_L4_PBGS | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop AAMDG, porphobilinogen synthase, EC 4.2.1.24 | Escherichia coli |
Mut_L4_PRAI_WT | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop GNEE, phosphoribosylanthranilate isomerase, EC 5.3.1.24 | Escherichia coli |
Mut_L4_TPS | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop LGQEDLH, thiamine-phosphate diphosphorylase, EC 2.5.1.3 | Escherichia coli |
Mut_L4_Ure | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop EDWGAT, urease, EC 3.5.1.5 | Escherichia coli |
Mut_L6_alphaTS | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 6, sequence of loop SRAGVTGAENRAALP, tryptophan synthase, EC 4.2.1.20 | Escherichia coli |
Mut_L6_DHDPS | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 6, sequence of loop TGNL, dihydrodipicolinate synthase, EC 4.2.1.52 | Escherichia coli |
Mut_L6_PBGS | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 6, sequence of loop PAGAY, porphobilinogen synthase, EC 4.2.1.24 | Escherichia coli |
Mut_L6_PRAI_WT | loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 6, sequence of loop NGQGGSGQRFD, phosphoribosylanthranilate isomerase, EC 5.3.1.24 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22900 | - |
PRAI-LoxP, His-tag, apparent molecular mass, protein concentration 2.0 microM | Escherichia coli |
22900 | - |
PRAI-LoxP, His-tag, apparent molecular mass, protein concentration 20 microM | Escherichia coli |
24100 | - |
PRAI-LoxP, theoretical | Escherichia coli |
49800 | - |
PRAI-LoxP, His-tag, apparent molecular mass, protein concentration 100 microM, deduced association state dimer | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00909 | - |
- |
Purification (Comment) | Organism |
---|---|
a nickel Sepharose column, HisTrap FF, and a gel-filtration column, Sephacryl S200, are used | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
phosphoribosylanthranilate isomerase | - |
Escherichia coli |
PRAI | - |
Escherichia coli |
PRAI-LoxP | variant of phosphoribosylanthranilate isomerase with an insertion coding for a Cre-lox recognition site in the loop linking alpha-helix 4 and beta-stand 5 | Escherichia coli |