Protein Variants | Comment | Organism |
---|---|---|
D127V | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermotoga maritima |
D127V/D169V | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermotoga maritima |
D127V/D173V | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermotoga maritima |
D130V | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermotoga maritima |
D130V/D176V | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermotoga maritima |
D169V | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermotoga maritima |
D173V | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermotoga maritima |
D176V | inactive | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.018 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D130V/D176V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.019 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D127V/D173V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.036 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D127V/D169V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.036 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D169V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.053 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D173V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.074 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D127V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.074 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D130V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(5-phospho-beta-D-ribosyl)anthranilate | Thermotoga maritima | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Thermotoga maritima | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphoribosylanthranilate isomerase | - |
Thermotoga maritima |
PRA isomerase | - |
Thermotoga maritima |
TrpF | - |
Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000183 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D130V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.000283 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D173V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.0012 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D130V/D176V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.009 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D127V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.028 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D169V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.032 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D127V/D173V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.522 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D127V/D169V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
3.7 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D130V/D176V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.019 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D127V/D173V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.036 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D127V/D169V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.036 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D169V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.053 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D173V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.074 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D127V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
0.074 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | mutant enzyme D130V, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima | |
13000 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C | Thermotoga maritima |