Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 35% residual activity | Methylococcus capsulatus | |
Cu2+ | 1 mM, 8% residual activity | Methylococcus capsulatus | |
Mg2+ | 5 mM, 30% loss of activity | Methylococcus capsulatus | |
additional information | at 1 mm sugar phosphate concentration, D-allulose 6-phosphate, D-fructose 6-phosphate, 6-phospho-D-gluconate, D-ribulose 5-phosphate, D-xylulose 5-phosphate, D-erythrose 4-phosphate and glyceraldehyde 3-phosphate do not affect the isomerase activity | Methylococcus capsulatus | |
Ni2+ | 1 mM, 14% residual activity | Methylococcus capsulatus | |
Zn2+ | 1 mM, 40% residual activity | Methylococcus capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
D-arabino-3-hexulose 6-phosphate | 30°C, pH 7.0 | Methylococcus capsulatus | |
1.1 | - |
D-fructose 6-phosphate | 30°C, pH 7.0 | Methylococcus capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for divalent cations | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
gel filtration | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
grown on methane | - |
Purification (Comment) | Organism |
---|---|
- |
Methylococcus capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1560 | - |
30°C, pH 7.0 | Methylococcus capsulatus |
Storage Stability | Organism |
---|---|
-15°C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months | Methylococcus capsulatus |
0-4°C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabino-3-hexulose 6-phosphate | - |
Methylococcus capsulatus | D-fructose 6-phosphate | - |
r | |
D-fructose 6-phosphate | - |
Methylococcus capsulatus | D-arabino-3-hexulose 6-phosphate | - |
r | |
additional information | enzyme is specific for substrates D-arabino-3-hexulose 6-phosphate and D-fructose 6-phosphate | Methylococcus capsulatus | ? | - |
? | |
additional information | no substrate: D-ribulose 5-phosphate, D-xylulose 5-phosphate or D-allulose 6-phosphate | Methylococcus capsulatus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, complete loss of activity | Methylococcus capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Methylococcus capsulatus |