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Literature summary for 5.3.1.27 extracted from

  • Ferenci, T.; Stroem, T.; Quayle, J.R.
    Purification and properties of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase from Methylococcus capsulatus (1974), Biochem. J., 144, 477-486.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 35% residual activity Methylococcus capsulatus
Cu2+ 1 mM, 8% residual activity Methylococcus capsulatus
Mg2+ 5 mM, 30% loss of activity Methylococcus capsulatus
additional information at 1 mm sugar phosphate concentration, D-allulose 6-phosphate, D-fructose 6-phosphate, 6-phospho-D-gluconate, D-ribulose 5-phosphate, D-xylulose 5-phosphate, D-erythrose 4-phosphate and glyceraldehyde 3-phosphate do not affect the isomerase activity Methylococcus capsulatus
Ni2+ 1 mM, 14% residual activity Methylococcus capsulatus
Zn2+ 1 mM, 40% residual activity Methylococcus capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
D-arabino-3-hexulose 6-phosphate 30°C, pH 7.0 Methylococcus capsulatus
1.1
-
D-fructose 6-phosphate 30°C, pH 7.0 Methylococcus capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for divalent cations Methylococcus capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
gel filtration Methylococcus capsulatus

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
grown on methane
-

Purification (Commentary)

Purification (Comment) Organism
-
Methylococcus capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1560
-
30°C, pH 7.0 Methylococcus capsulatus

Storage Stability

Storage Stability Organism
-15°C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months Methylococcus capsulatus
0-4°C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabino-3-hexulose 6-phosphate
-
Methylococcus capsulatus D-fructose 6-phosphate
-
r
D-fructose 6-phosphate
-
Methylococcus capsulatus D-arabino-3-hexulose 6-phosphate
-
r
additional information enzyme is specific for substrates D-arabino-3-hexulose 6-phosphate and D-fructose 6-phosphate Methylococcus capsulatus ?
-
?
additional information no substrate: D-ribulose 5-phosphate, D-xylulose 5-phosphate or D-allulose 6-phosphate Methylococcus capsulatus ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
10 min, complete loss of activity Methylococcus capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
Methylococcus capsulatus