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Literature summary for 5.3.1.3 extracted from
- Characterization of a recombinant L-fucose isomerase from Caldicellulosiruptor saccharolyticus that isomerizes L-fucose, D-arabinose, D-altrose, and L-galactose (2010), Biotechnol. Lett., 32, 299-304.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pGEM-T Easy, and subsequently into the vector pET15b for expression in Escherichia coli ER2566 cells | Caldicellulosiruptor saccharolyticus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 61 | - |
D-altrose | - |
Caldicellulosiruptor saccharolyticus |  |
| 152 | - |
D-arabinose | - |
Caldicellulosiruptor saccharolyticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Caldicellulosiruptor saccharolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
determined by SDS-PAGE | Caldicellulosiruptor saccharolyticus |
| 68110 | - |
theoretical | Caldicellulosiruptor saccharolyticus |
| 204000 | - |
homotrimer, determined by gel filtration | Caldicellulosiruptor saccharolyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-altrose | Caldicellulosiruptor saccharolyticus | - |
D-psicose | - |
? | |
| D-arabinose | Caldicellulosiruptor saccharolyticus | - |
D-ribulose | - |
? | |
| L-galactose | Caldicellulosiruptor saccharolyticus | - |
L-tagatose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor saccharolyticus | A4XJ56 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| on a His-Trap HP column | Caldicellulosiruptor saccharolyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | - |
substrate L-galactose | Caldicellulosiruptor saccharolyticus |
| 15 | - |
substrate D-altrose | Caldicellulosiruptor saccharolyticus |
| 62 | - |
substrate D-arabinose | Caldicellulosiruptor saccharolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-altrose | - |
Caldicellulosiruptor saccharolyticus | D-psicose | - |
? | |
| D-arabinose | - |
Caldicellulosiruptor saccharolyticus | D-ribulose | - |
? | |
| L-galactose | - |
Caldicellulosiruptor saccharolyticus | L-tagatose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 68000 Da | Caldicellulosiruptor saccharolyticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-Arabinose isomerase | L-fucose isomerase, bifunctional enzyme | Caldicellulosiruptor saccharolyticus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0287 | - |
D-altrose | - |
Caldicellulosiruptor saccharolyticus | |
| 0.1823 | - |
D-arabinose | - |
Caldicellulosiruptor saccharolyticus | |
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