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Literature summary for 5.3.1.4 extracted from
- Identification and characterization of a novel L-arabinose isomerase from Anoxybacillus flavithermus useful in D-tagatose production (2011), Extremophiles, 15, 441-450.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Borate | the catalytic efficiency (kcat/Km) for D-galactose with borate is twice as much as that without borate | Anoxybacillus flavithermus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Anoxybacillus flavithermus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | about 85% residual activity at 1 mM | Anoxybacillus flavithermus | |
| Cu2+ | about 25% residual activity at 1 mM | Anoxybacillus flavithermus | |
| Fe3+ | about 35% residual activity at 1 mM | Anoxybacillus flavithermus | |
| Li+ | about 90% residual activity at 1 mM | Anoxybacillus flavithermus | |
| Mg2+ | about 90% residual activity at 1 mM | Anoxybacillus flavithermus | |
| Mn2+ | about 70% residual activity at 1 mM | Anoxybacillus flavithermus | |
| Na+ | about 90% residual activity at 1 mM | Anoxybacillus flavithermus | |
| Zn2+ | about 65% residual activity at 1 mM | Anoxybacillus flavithermus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 25.19 | - |
D-galactose | without borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
| 26.81 | - |
D-galactose | with 10 mM borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
| 78.5 | - |
L-arabinose | in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ni2+ | about 135% activity at 1 mM | Anoxybacillus flavithermus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55876 | - |
x * 55876, calculated from amino acid sequence | Anoxybacillus flavithermus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anoxybacillus flavithermus | F6KRI7 | - |
- |
| Anoxybacillus flavithermus TC-06 | F6KRI7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Anoxybacillus flavithermus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, glycine-NaOH buffer (pH 9.5), 60 days, no loss of activity | Anoxybacillus flavithermus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | the enzyme has high preference for D-galactose (100% activity) | Anoxybacillus flavithermus | D-tagatose | - |
? | |
| D-galactose | the enzyme has high preference for D-galactose (100% activity) | Anoxybacillus flavithermus TC-06 | D-tagatose | - |
? | |
| D-Xylose | 32.3% activity compared to D-galactose | Anoxybacillus flavithermus | ? | - |
? | |
| D-Xylose | 32.3% activity compared to D-galactose | Anoxybacillus flavithermus TC-06 | ? | - |
? | |
| L-arabinose | 40% activity compared to D-galactose | Anoxybacillus flavithermus | L-ribulose | - |
? | |
| L-arabinose | 40% activity compared to D-galactose | Anoxybacillus flavithermus TC-06 | L-ribulose | - |
? | |
| additional information | D-glucose and D-allose do not serve as substrate | Anoxybacillus flavithermus | ? | - |
? | |
| additional information | D-glucose and D-allose do not serve as substrate | Anoxybacillus flavithermus TC-06 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55876, calculated from amino acid sequence | Anoxybacillus flavithermus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-AI | - |
Anoxybacillus flavithermus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Anoxybacillus flavithermus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 85 | the enzyme is not active below 75°C, the relative activity is less than 50% below 85°C | Anoxybacillus flavithermus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
L-arabinose | in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
| 2.2 | - |
D-galactose | without borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
| 78.5 | - |
D-galactose | with 10 mM borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | 10.5 | - |
Anoxybacillus flavithermus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | the enzyme is not active below pH 7.0, the relative activity is less than 50% below pH 9.5 | Anoxybacillus flavithermus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Anoxybacillus flavithermus | calculated from amino acid sequence | - |
5.1 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
L-arabinose | in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
| 0.09 | - |
D-galactose | without borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
| 0.16 | - |
D-galactose | with 10 mM borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
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