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Literature summary for 5.3.1.5 extracted from

  • Inyang, C.U.; Gebhart, U.; Obi, S.K.C.; Bisswanger, H.
    Isolation and characterization of a D-glucose/xylose isomerase from a new thermophilic strain Streptomyces sp. (PLC) (1995), Appl. Microbiol. Biotechnol., 43, 632-638.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Streptomyces sp.
D-sorbitol
-
Streptomyces sp.
D-xylitol competitive Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
35
-
D-xylose
-
Streptomyces sp.
400
-
D-glucose
-
Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ two metal ions bind per subunit to non-identical sites. Mg2+, Mn2+, and Co2+ are of comparable efficiency for the D-xylose isomerization. Co2+ is the most efficient cofactor for D-glucose isomerization Streptomyces sp.
Mg2+ two metal ions bind per subunit to non-identical sites. Mg2+, Mn2+, and Co2+ are of comparable efficiency for the D-xylose isomerization Streptomyces sp.
Mn2+ two metal ions bind per subunit to non-identical sites. Mg2+, Mn2+, and Co2+ are of comparable efficiency for the D-xylose isomerization Streptomyces sp.
Zn2+ weak cofactor for D-glucose isomerization Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
183000
-
sucrose density gradient centrifugation Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
thermophilic strain PLC
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4
-
-
Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose
-
Streptomyces sp. D-Fructose
-
?
D-Xylose
-
Streptomyces sp. D-Xylulose
-
?

Subunits

Subunits Comment Organism
tetramer
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
78
-
D-glucose, activity increases up to 85°C and remains constant nearly to 100°C Streptomyces sp.
85
-
D-xylose,activity increases up to 85°C and remains constant nearly to 100°C Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
half life in presence of Co2+: 7 days, half-life in presence of Mg2+: 9 days, in presence of Mn2+ the enzyme activity remains constant for at least 10 days Streptomyces sp.
70
-
in presence of Mn2+, 50% loss of activity after 5 days Streptomyces sp.
98
-
half-life: 15 min Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Streptomyces sp.