Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Streptomyces sp. | |
D-sorbitol | - |
Streptomyces sp. | |
D-xylitol | competitive | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35 | - |
D-xylose | - |
Streptomyces sp. | |
400 | - |
D-glucose | - |
Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | two metal ions bind per subunit to non-identical sites. Mg2+, Mn2+, and Co2+ are of comparable efficiency for the D-xylose isomerization. Co2+ is the most efficient cofactor for D-glucose isomerization | Streptomyces sp. | |
Mg2+ | two metal ions bind per subunit to non-identical sites. Mg2+, Mn2+, and Co2+ are of comparable efficiency for the D-xylose isomerization | Streptomyces sp. | |
Mn2+ | two metal ions bind per subunit to non-identical sites. Mg2+, Mn2+, and Co2+ are of comparable efficiency for the D-xylose isomerization | Streptomyces sp. | |
Zn2+ | weak cofactor for D-glucose isomerization | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
183000 | - |
sucrose density gradient centrifugation | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
thermophilic strain PLC | - |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.4 | - |
- |
Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucose | - |
Streptomyces sp. | D-Fructose | - |
? | |
D-Xylose | - |
Streptomyces sp. | D-Xylulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
78 | - |
D-glucose, activity increases up to 85°C and remains constant nearly to 100°C | Streptomyces sp. |
85 | - |
D-xylose,activity increases up to 85°C and remains constant nearly to 100°C | Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
half life in presence of Co2+: 7 days, half-life in presence of Mg2+: 9 days, in presence of Mn2+ the enzyme activity remains constant for at least 10 days | Streptomyces sp. |
70 | - |
in presence of Mn2+, 50% loss of activity after 5 days | Streptomyces sp. |
98 | - |
half-life: 15 min | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptomyces sp. |