Literature summary for 5.3.1.5 extracted from

Lee, C.; Bagdasarian, M.; Meng, M.; Zeikus, J.G.
Catalytic mechanism of xylose (glucose) isomerase from Clostridium thermosulfurogenes. Characterization of the structural gene and function of active site histidine (1990), J. Biol. Chem., 265, 19082-19090.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, sequence homology with enzymes from other sources	Thermoanaerobacterium thermosulfurigenes

Protein Variants

Protein Variants	Comment	Organism
H101F	substitution of His101 by Phe completely abolishes enzyme activity. When His101 is changed to Glu, Gln, Asn, or Asp, approximately 10-16% of wild-type enzyme activity is retained by the mutant enzymes. The His101Gln mutant enzyme is resistant to diethyldicarbonate inhibition which completely inactivates the wild-type enzyme	Thermoanaerobacterium thermosulfurigenes

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
130	-	D-glucose	D-glucose, mutant enzyme His71Phe	Thermoanaerobacterium thermosulfurigenes
138	-	D-glucose	mutant enzyme His101Gln	Thermoanaerobacterium thermosulfurigenes
142	-	D-glucose	glucose, wild-type enzyme	Thermoanaerobacterium thermosulfurigenes
152	-	D-glucose	mutant enzyme His152Phe	Thermoanaerobacterium thermosulfurigenes
168	-	D-glucose	mutant enzyme His101Asn	Thermoanaerobacterium thermosulfurigenes
198	-	D-glucose	mutant enzyme His101Asp	Thermoanaerobacterium thermosulfurigenes
252	-	D-glucose	mutant enzyme His101Glu	Thermoanaerobacterium thermosulfurigenes

Organism

Organism	UniProt	Comment	Textmining
Thermoanaerobacterium thermosulfurigenes	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Thermoanaerobacterium thermosulfurigenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Glucose	-	Thermoanaerobacterium thermosulfurigenes	D-Fructose	-	?
D-Xylose	-	Thermoanaerobacterium thermosulfurigenes	D-Xylulose	-	?

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Release 2023.1 (January 2023)