Application | Comment | Organism |
---|---|---|
energy production | engineering of Saccharomyces cerevisiae for alcoholic fermentation of D-xylose | Thermus thermophilus |
energy production | engineering Saccharomyces cerevisiae for alcoholic fermentation of D-xylose | Piromyces sp. |
Cloned (Comment) | Organism |
---|---|
Saccharomyces cerevisiae is transformed with a gene encoding xylose isomerase (Thermus thermophilus) | Thermus thermophilus |
Saccharomyces cerevisiae is transformed with a gene encoding xylose isomerase originating from Piromyces sp. | Piromyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Saccharomyces cerevisiae: five enzymes of non-oxidative pentose-phosphate-pathway are induced, a non-specific aldose reductase is deleted | Thermus thermophilus |
additional information | Saccharomyces cerevisiae: five enzymes of non-oxidative pentose-phosphate-pathway are induced, a unspecific aldose reductase is deleted | Piromyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | depends on the origin of the enzyme | Thermus thermophilus | |
Co2+ | depends on the origin of the enzyme | Piromyces sp. | |
Mg2+ | depends on the origin of the enzyme | Thermus thermophilus | |
Mg2+ | depends on the origin of the enzyme | Piromyces sp. | |
Mn2+ | depends on the origin of the enzyme | Thermus thermophilus | |
Mn2+ | depends on the origin of the enzyme | Piromyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | Thermus thermophilus | - |
D-Xylulose | - |
r | |
D-Xylose | Piromyces sp. | - |
D-Xylulose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Saccharomyces cerevisiae | - |
- |
- |
Piromyces sp. | - |
source of xylose isomerase gene, a phylogenetic tree of the diversity of xylose isomerase genes is given - based on GenBank database | - |
Thermus thermophilus | - |
source of xylose isomerase gene, a phylogenetic tree of the diversity of xylose isomerase genes is given - based on GenBank database | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
measurement of the degradation of xylose and glucose | Thermus thermophilus |
additional information | - |
measurement of the degradation of xylose and glucose | Piromyces sp. |
additional information | - |
measurement of the production of ethanol, glycerol, organic acids, xylitol, CO2 | Thermus thermophilus |
additional information | - |
measurement of the production of ethanol, glycerol, organic acids, xylitol, CO2 | Piromyces sp. |
additional information | - |
strain is cultivated under aerobic and anaerobic conditions | Thermus thermophilus |
additional information | - |
strains are cultivated under aerobic and anaerobic conditions | Piromyces sp. |
0.017 | - |
Saccharomyces cerevisiae overexprssing five own enzymes of pentose phosphate pathway, deletion of an unspecific aldose reductase, overexpressing xylose isomerase from Piromyces sp., 30°C | Piromyces sp. |
0.04 | - |
Saccharomyces cerevisiae overexpressing xylose isomerase from Thermus thermophilus, 30°C | Thermus thermophilus |
1 | - |
Saccharomyces cerevisiae overexpressing xylose isomerase from Thermus thermophilus, 85°C | Thermus thermophilus |
1.1 | - |
Saccharomyces cerevisiae overexprssing xylose isomerase from Piromyces sp., 30°C | Piromyces sp. |
1.1 | - |
Saccharomyces cerevisiae strain CEN.PK overexprssing xylose isomerase (xylA gene) from Piromyces sp., TPI1 promoter, 30°C | Piromyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucose | - |
Thermus thermophilus | D-Fructose | - |
r | |
D-Glucose | - |
Piromyces sp. | D-Fructose | - |
r | |
D-Xylose | - |
Thermus thermophilus | D-Xylulose | - |
r | |
D-Xylose | - |
Piromyces sp. | D-Xylulose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
D-xylose ketol isomerase | - |
Thermus thermophilus |
D-xylose ketol isomerase | - |
Piromyces sp. |
xylose isomerase | - |
Thermus thermophilus |
xylose isomerase | - |
no activity in Saccharomyces cerevisiae |
xylose isomerase | - |
Piromyces sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | - |
Thermus thermophilus |
60 | 80 | - |
Piromyces sp. |