Protein Variants | Comment | Organism |
---|---|---|
K32A | 15fold decrease in Ki-value for the competitive inhibitor, (E)-2-fluoro-p-hydroxycinnamate compared to wild-type enzyme, turnover number for enol-phenylpyruvate is 9% of that for the wild-type enzyme, turnover number for enol-(p-hydroxyphenyl)pyruvate is 11% of that for the wild-type enzyme, the ratio of turnover number and Km-value for enol-phenylpyruvate is 8% of that for the wild-type enzyme, the ratio of turnover number and KM-value for enol(p-hydroxyphenyl)pyruvate is 16% of the value for the wild-type enzyme | Mus musculus |
K32R | modest decrease in the stereoselectivity of the reaction and in the binding affinity of the competitive inhibitor, (E)-2-fluoro-p-hydroxycinnamate, turnover number for enol-phenylpyruvate is 47% of that for the wild-type enzyme, turnover number for enol-(p-hydroxyphenyl)pyruvate is 110% of that for the wild-type enzyme, the ratio of turnover number and Km-value for enol-phenylpyruvate or enol(p-hydroxyphenyl)pyruvate is about 70% of the value for the wild-type enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E)-2-fluoro-p-hydroxycinnamate | competitive | Mus musculus | |
4-coumaric acid | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
enol-phenylpyruvate | 23°C, pH 6.5, mutant enzyme K32R | Mus musculus | |
0.14 | - |
enol-(p-hydroxyphenyl)pyruvate | 23°C, pH 6.5, mutant enzyme K32A | Mus musculus | |
0.15 | - |
enol-phenylpyruvate | 23°C, pH 6.5, wild-type enzyme | Mus musculus | |
0.18 | - |
enol-phenylpyruvate | 23°C, pH 6.5, mutant enzyme K32A | Mus musculus | |
0.2 | - |
enol-(p-hydroxyphenyl)pyruvate | 23°C, pH 6.5, wild-type enzyme | Mus musculus | |
0.35 | - |
enol-(p-hydroxyphenyl)pyruvate | 23°C, pH 6.5, mutant enzyme K32R | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12316 | - |
x * 12316, mutant enzyme K32A, electrospray ionization mass spectrometry | Mus musculus |
12371 | - |
x * 12371, wild-type enzyme, electrospray ionization mass spectrometry | Mus musculus |
12401 | - |
x * 12401, mutant enzyme K32R, electrospray ionization mass spectrometry | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
enol-(p-hydroxyphenyl)pyruvate | - |
Mus musculus | keto-(p-hydroxyphenyl)pyruvate | - |
r | |
enol-phenylpyruvate | - |
Mus musculus | keto-phenylpyruvate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 12316, mutant enzyme K32A, electrospray ionization mass spectrometry | Mus musculus |
? | x * 12371, wild-type enzyme, electrospray ionization mass spectrometry | Mus musculus |
? | x * 12401, mutant enzyme K32R, electrospray ionization mass spectrometry | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
Macrophage migration inhibitory factor | - |
Mus musculus |
MIF | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
enol-(p-hydroxyphenyl)pyruvate | 23°C, pH 6.5, mutant enzyme K32A | Mus musculus | |
30 | - |
enol-phenylpyruvate | 23°C, pH 6.5, mutant enzyme K32A | Mus musculus | |
140 | - |
enol-(p-hydroxyphenyl)pyruvate | 23°C, pH 6.5, wild-type enzyme | Mus musculus | |
150 | - |
enol-phenylpyruvate | 23°C, pH 6.5, mutant enzyme K32R | Mus musculus | |
160 | - |
enol-(p-hydroxyphenyl)pyruvate | 23°C, pH 6.5, mutant enzyme K32R | Mus musculus | |
320 | - |
enol-phenylpyruvate | 23°C, pH 6.5, wild-type enzyme | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
(E)-2-fluoro-p-hydroxycinnamate | 23°C, pH 6.5, mutant enzyme K32R | Mus musculus | |
0.04 | - |
(E)-2-fluoro-p-hydroxycinnamate | 23°C, pH 6.5, mutant enzyme K32A | Mus musculus | |
0.234 | - |
4-coumaric acid | 23°C, pH 6.5, mutant enzyme K32R | Mus musculus | |
0.375 | - |
4-coumaric acid | 23°C, pH 6.5, mutant enzyme K32A | Mus musculus |