Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.2.1 extracted from

  • Golubkov, P.A.; Johnson, W.H.; Czerwinski, R.M.; Person, M.D.; Wang, S.C.; Whitman, C.P.; Hackert, M.L.
    Inactivation of the phenylpyruvate tautomerase activity of macrophage migration inhibitory factor by 2-oxo-4-phenyl-3-butynoate (2006), Bioorg. Chem., 34, 183-199.
    View publication on PubMed
Search for more literature for 5.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals are soaked with inhibitor 2-oxo-4-phenyl-3-butynoate Mus musculus

Protein Variants

Protein Variants Comment Organism
E16A kinetics similar to wild-type Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
2-oxo-4-phenyl-3-butynoate irreversible inactivation by covalent modifiaction of N-terminal proline residue via a Michael-addition of proline to C4 of the inhibitor Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 4-(hydroxyphenyl)pyruvate mutant E16A, pH 6.8 Mus musculus
0.16
-
 enol-phenylpyruvate mutant E16A, pH 6.8 Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P34884
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(hydroxyphenyl)pyruvate enol form Mus musculus 4-(hydroxyphenyl)pyruvate keto form ?
enol-phenylpyruvate
-
 Mus musculus keto-phenylpyruvate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60
-
 4-(hydroxyphenyl)pyruvate mutant E16A, pH 6.8 Mus musculus
200
-
 enol-phenylpyruvate mutant E16A, pH 6.8 Mus musculus