Literature summary for 5.3.3.18 extracted from

Grishin, A.M.; Ajamian, E.; Zhang, L.; Rouiller, I.; Bostina, M.; Cygler, M.
Protein-protein interactions in the beta-oxidation part of the phenylacetate utilization pathway: crystal structure of the PaaF-PaaG hydratase-isomerase complex (2012), J. Biol. Chem., 287, 37986-37996.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.1 M sodium citrate, pH 5.5, 0.2 M sodium acetate, and 5% (w/w) PEG 4000	Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28779	-	3 * 28779, MALDI-TOF mass spectrometry	Thermus thermophilus
30000	-	3 * 30000, SDS-PAGE	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA	Thermus thermophilus	-	2-oxepin-2(3H)-ylideneacetyl-CoA	-	r

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA beads chromatography and Superdex 200 gel filtration	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA	-	Thermus thermophilus	2-oxepin-2(3H)-ylideneacetyl-CoA	-	r

Subunits

Subunits	Comment	Organism
homotrimer	3 * 30000, SDS-PAGE	Thermus thermophilus
homotrimer	3 * 28779, MALDI-TOF mass spectrometry	Thermus thermophilus

Synonyms

Synonyms	Comment	Organism
paaG	-	Thermus thermophilus

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Release 2023.1 (January 2023)