Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FMN | required as cofactor. The enzyme requires the combined presence of FMN, NADPH and Mg2+ | Methanothermobacter thermautotrophicus | |
NADPH | required as cofactor. The enzyme requires the combined presence of FMN, NADPH and Mg2+ | Methanothermobacter thermautotrophicus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, His6-tagged type II enzyme | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required. The enzyme requires the combined presence of FMN, NADPH and Mg2+ | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
4 * 48000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | Methanothermobacter thermautotrophicus | the enzyme catalyzes the interconversion of the fundamental five carbon homoallylic and allylic diphosphate building blocks required for biosynthesis of isoprenoid compounds | dimethylallyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
type II isopentenyl diphosphate isomerase | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus | dimethylallyl diphosphate | - |
? | |
isopentenyl diphosphate | the enzyme catalyzes the interconversion of the fundamental five carbon homoallylic and allylic diphosphate building blocks required for biosynthesis of isoprenoid compounds | Methanothermobacter thermautotrophicus | dimethylallyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 48000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Methanothermobacter thermautotrophicus |