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Literature summary for 5.3.3.2 extracted from
- Identification of an archaeal type II isopentenyl diphosphate isomerase in Methanothermobacter thermautotrophicus (2004), J. Bacteriol., 186, 1811-1817.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FMN | required as cofactor. The enzyme requires the combined presence of FMN, NADPH and Mg2+ | Methanothermobacter thermautotrophicus |  |
| NADPH | required as cofactor. The enzyme requires the combined presence of FMN, NADPH and Mg2+ | Methanothermobacter thermautotrophicus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, His6-tagged type II enzyme | Methanothermobacter thermautotrophicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.064 | - |
isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required. The enzyme requires the combined presence of FMN, NADPH and Mg2+ | Methanothermobacter thermautotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
4 * 48000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isopentenyl diphosphate | Methanothermobacter thermautotrophicus | the enzyme catalyzes the interconversion of the fundamental five carbon homoallylic and allylic diphosphate building blocks required for biosynthesis of isoprenoid compounds | dimethylallyl diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | - |
type II isopentenyl diphosphate isomerase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus | dimethylallyl diphosphate | - |
? | |
| isopentenyl diphosphate | the enzyme catalyzes the interconversion of the fundamental five carbon homoallylic and allylic diphosphate building blocks required for biosynthesis of isoprenoid compounds | Methanothermobacter thermautotrophicus | dimethylallyl diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 48000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Methanothermobacter thermautotrophicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Methanothermobacter thermautotrophicus |
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