Cloned (Comment) | Organism |
---|---|
into a pET-vector for expression in Escherichia coli Bl21DE3 cells | Saccharolobus shibatae |
Crystallization (Comment) | Organism |
---|---|
the crystal structures of the substrate-free enzyme and of the substrate-enzyme complexes, in the oxidized and reduced states, are solved to resolutions between 1.99 and 3.1 A, six distinct types of type 2 IDI crystals are obtained | Saccharolobus shibatae |
Protein Variants | Comment | Organism |
---|---|---|
D216A | mutation of highly conserved residue | Saccharolobus shibatae |
E161A | mutation of highly conserved residue | Saccharolobus shibatae |
E194A | mutation of highly conserved residue | Saccharolobus shibatae |
E229A | mutation of highly conserved residue | Saccharolobus shibatae |
H11A | mutation of highly conserved residue | Saccharolobus shibatae |
H155A | mutation of highly conserved residue | Saccharolobus shibatae |
K193A | mutation of highly conserved residue | Saccharolobus shibatae |
K8A | mutation of highly conserved residue | Saccharolobus shibatae |
N125A | mutation of highly conserved residue | Saccharolobus shibatae |
N157A | mutation of highly conserved residue | Saccharolobus shibatae |
Q160A | mutation of highly conserved residue | Saccharolobus shibatae |
R232A | mutation of highly conserved residue | Saccharolobus shibatae |
R7A | mutation of highly conserved residue | Saccharolobus shibatae |
S96A | mutation of highly conserved residue | Saccharolobus shibatae |
T68A | mutation of highly conserved residue | Saccharolobus shibatae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharolobus shibatae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | Saccharolobus shibatae | - |
dimethylallyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | P61615 | - |
- |
Purification (Comment) | Organism |
---|---|
on a HisTrap column, for crystallisation, the enzyme is loaded on a HiLoad 16/60 Superdex 200 column | Saccharolobus shibatae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
mutant D216A, relative IPP isomerase activity 35%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant E161A, relative IPP isomerase activity 0.5%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant E194A, relative IPP isomerase activity 15%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant E229A, relative IPP isomerase activity 6.5%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant H11A, relative IPP isomerase activity 4%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant H155A, relative IPP isomerase activity 3%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant K193A, relative IPP isomerase activity 1%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant K8A, relative IPP isomerase activity 0.1%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant N125A, relative IPP isomerase activity 5%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant N157A, relative IPP isomerase activity 0.5%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant Q160A, relative IPP isomerase activity 0.1%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant R232A, relative IPP isomerase activity 2%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant R7A, relative IPP isomerase activity 0.1%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant S96A, relative IPP isomerase activity 3%, wild-type enzyme 100% | Saccharolobus shibatae |
additional information | - |
mutant T68A, relative IPP isomerase activity 6%, wild-type enzyme 100% | Saccharolobus shibatae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | - |
Saccharolobus shibatae | dimethylallyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDI | - |
Saccharolobus shibatae |
IPP isomerase | - |
Saccharolobus shibatae |
isopentenyl diphosphate:dimethylallyl diphosphate isomerase | - |
Saccharolobus shibatae |
type 2 isopentenyl-diphosphate isomerase | - |
Saccharolobus shibatae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Saccharolobus shibatae | |
NAD(P)H | used only for the reduction of FMN, can be replaced with Na2S2O4 | Saccharolobus shibatae |