Literature summary for 5.3.3.2 extracted from

Sun, J.; Zhang, Y.; Liu, H.; Zou, Z.; Zhang, C.; Zhang, X.; Li, H.; Ye, Z.
A novel cytoplasmic isopentenyl diphosphate isomerase gene from tomato (Solanum lycopersicum): Cloning, expression, and color complementation (2010), Plant Mol. Biol. Rep., 28, 473-480.

No PubMed abstract available

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Application

Application	Comment	Organism
biotechnology	overexpression of SlIPI in engineered Escherichia coli can promote the biosynthesis of beta-carotene in bacteria, the result provides a foundation for terpenoid metabolic engineering using the SlIPI gene	Solanum lycopersicum

Cloned(Commentary)

Cloned (Comment)	Organism
into the vector pMD18-T for sequencing, and subsequently into pET-28a+ for expression in Escherichia coli BL21DE3 cells	Solanum lycopersicum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Solanum lycopersicum	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27180	-	theoretical	Solanum lycopersicum
30000	-	determined by SDS-PAGE	Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
isopentenyl diphosphate	Solanum lycopersicum	-	dimethylallyl diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Solanum lycopersicum	A9LRT7	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower	-	Solanum lycopersicum	-
fruit	-	Solanum lycopersicum	-
leaf	cDNA clone of the tomato IPI gene is amplified using cDNA from young leaves as template	Solanum lycopersicum	-
root	highest expression level	Solanum lycopersicum	-
stem	-	Solanum lycopersicum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isopentenyl diphosphate	-	Solanum lycopersicum	dimethylallyl diphosphate	-	r

Synonyms

Synonyms	Comment	Organism
IPI	-	Solanum lycopersicum
isopentenyl diphosphate isomerase	-	Solanum lycopersicum
SlIPI	-	Solanum lycopersicum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Solanum lycopersicum	theoretical	-	5.06

General Information

General Information	Comment	Organism
physiological function	isopentenyl diphosphate and dimethylallyl diphosphate are essential precursors for terpenoid biosynthesis	Solanum lycopersicum

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Release 2023.1 (January 2023)