Cloned (Comment) | Organism |
---|---|
recombinantly expressed with a polyhistidine tag at its N terminus in Escherichia coli BL21 | Saccharolobus shibatae |
Crystallization (Comment) | Organism |
---|---|
crystallized at 20°C using the hanging-drop vapor diffusion method with a reservoir solution containing 0.1 M Tris-HCl (pH 8.0), 0.2 M sodium citrate, and 30% (vol/vol) polyethylene glycol 400 (PEG 400) | Saccharolobus shibatae |
Protein Variants | Comment | Organism |
---|---|---|
E13R/R235E | the mutant and the wild-type enzyme show similar Vmax values, while the Km of E13R/R235E is smaller than that of the wild type. The mutant is in the tetrameric state even at a concentration where the wild-type enzyme dominantly forms an octamer | Saccharolobus shibatae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00714 | - |
isopentenyl diphosphate | pH 6.0, 60°C, mutant enzyme E13R/R235E | Saccharolobus shibatae | |
0.0463 | - |
isopentenyl diphosphate | pH 6.0, 60°C, wild-type enzyme | Saccharolobus shibatae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | Saccharolobus shibatae | - |
dimethylallyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | P61615 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus shibatae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | - |
Saccharolobus shibatae | dimethylallyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | substrate binding causes the dissociation of an octamer into tetramers. The mutant enzyme E13R/R235E is in the tetrameric state even at a concentration where the wild-type enzyme dominantly forms an octamer | Saccharolobus shibatae |
Synonyms | Comment | Organism |
---|---|---|
type 2 isopentenyl diphosphate isomerase | - |
Saccharolobus shibatae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus shibatae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Saccharolobus shibatae |