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Literature summary for 5.3.3.2 extracted from

  • Sen, S.E.; Tomasello, A.; Grasso, M.; Denton, R.; Macor, J.; Beliveau, C.; Cusson, M.; Crowell, D.N.
    Cloning, expression and characterization of lepidopteran isopentenyl diphosphate isomerase (2012), Insect Biochem. Mol. Biol., 42, 739-750.
    View publication on PubMed
Search for more literature for 5.3.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells Manduca sexta
expressed in Escherichia coli Rosetta cells Choristoneura fumiferana

Protein Variants

Protein Variants Comment Organism
C113S inactive Choristoneura fumiferana
C113S/114S inactive Choristoneura fumiferana
C114S inactive Choristoneura fumiferana
E169/171Q inactive Choristoneura fumiferana
E169Q inactive Choristoneura fumiferana
E171Q inactive Choristoneura fumiferana
W216F 10% activity compared to the wild type enzyme Choristoneura fumiferana
Y105F 115% activity compared to the wild type enzyme Choristoneura fumiferana

Inhibitors

Inhibitors Comment Organism Structure
(2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate
-
 Choristoneura fumiferana
(2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate
-
 Manduca sexta
(2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate
-
 Sus scrofa
(2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate
-
 Choristoneura fumiferana
(2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate
-
 Manduca sexta
(2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate
-
 Sus scrofa
(2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate
-
 Choristoneura fumiferana
(2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate
-
 Manduca sexta
(2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate
-
 Sus scrofa
2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate
-
 Choristoneura fumiferana
2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate
-
 Manduca sexta
2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate
-
 Sus scrofa
2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate
-
 Choristoneura fumiferana
2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate
-
 Manduca sexta
dimethylallyl diphosphate
-
 Choristoneura fumiferana
dimethylallyl diphosphate
-
 Manduca sexta
dimethylallyl diphosphate
-
 Sus scrofa
dimethylammonium diphosphate
-
 Choristoneura fumiferana
dimethylammonium diphosphate
-
 Manduca sexta
dimethylammonium diphosphate
-
 Sus scrofa
homodimethylallyl diphosphate
-
 Choristoneura fumiferana
homodimethylallyl diphosphate
-
 Manduca sexta
homodimethylallyl diphosphate
-
 Sus scrofa
homoisopentenyl diphosphate
-
 Choristoneura fumiferana
homoisopentenyl diphosphate
-
 Manduca sexta
homoisopentenyl diphosphate
-
 Sus scrofa
additional information not inhibited by 2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
 isopentenyl diphosphate wild type enzyme, in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana
0.006
-
 isopentenyl diphosphate mutant enzyme E169Q, in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana
0.006
-
 isopentenyl diphosphate mutant enzyme Y105F, in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana
0.0116
-
 isopentenyl diphosphate mutant enzyme W216F, in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana
0.0128
-
 isopentenyl diphosphate mutant enzyme Y159F, in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Sus scrofa 5739
-
mitochondrion
-
 Manduca sexta 5739
-
mitochondrion
-
 Choristoneura fumiferana 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ preference for Mn2+ over Mg2+ Sus scrofa
Mg2+ preference for Mn2+ over Mg2+ Manduca sexta
Mg2+ preference for Mn2+ over Mg2+ Choristoneura fumiferana
Mn2+ preference for Mn2+ over Mg2+ Sus scrofa
Mn2+ preference for Mn2+ over Mg2+ Manduca sexta
Mn2+ preference for Mn2+ over Mg2+ Choristoneura fumiferana
additional information not activated by Zn2*, Fe2*, or Cu2+ Choristoneura fumiferana
additional information not activated by Zn2+, Fe2+, or Cu2+ Manduca sexta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29200
-
 x * 29200, calculated from amino acid sequence Manduca sexta
29200
-
 x * 29200, calculated from amino acid sequence Choristoneura fumiferana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isopentenyl diphosphate Sus scrofa
-
 dimethylallyl diphosphate
-
 ?
isopentenyl diphosphate Manduca sexta
-
 dimethylallyl diphosphate
-
 ?
isopentenyl diphosphate Choristoneura fumiferana
-
 dimethylallyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Choristoneura fumiferana
-
-
-
Manduca sexta
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography Manduca sexta
TALON metal affinity resin column chromatography Choristoneura fumiferana

Source Tissue

Source Tissue Comment Organism Textmining
corpus allatum highly abundant in the corpora allata Manduca sexta
-
liver
-
 Sus scrofa
-
midgut
-
 Choristoneura fumiferana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopentenyl diphosphate
-
 Sus scrofa dimethylallyl diphosphate
-
 ?
isopentenyl diphosphate
-
 Manduca sexta dimethylallyl diphosphate
-
 ?
isopentenyl diphosphate
-
 Choristoneura fumiferana dimethylallyl diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 29200, calculated from amino acid sequence Manduca sexta
? x * 29200, calculated from amino acid sequence Choristoneura fumiferana

Synonyms

Synonyms Comment Organism
IPPI
-
 Sus scrofa
IPPI
-
 Manduca sexta
IPPI
-
 Choristoneura fumiferana
isopentenyl diphosphate isomerase
-
 Sus scrofa
isopentenyl diphosphate isomerase
-
 Manduca sexta
isopentenyl diphosphate isomerase
-
 Choristoneura fumiferana

pI Value

Organism Comment pI Value Maximum pI Value
Manduca sexta calculated from amino acid sequence
-
 6
Choristoneura fumiferana calculated from amino acid sequence
-
 6.4

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0008
-
 in 50 mM Tris-HCl (pH 7.0), at 37°C Sus scrofa dimethylammonium diphosphate
0.003
-
 in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana 2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate
0.0055
-
 in 50 mM Tris-HCl (pH 7.0), at 37°C Sus scrofa 2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate
0.007
-
 in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana 2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate
0.066
-
 in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana homoisopentenyl diphosphate
0.092
-
 in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana dimethylallyl diphosphate
0.131
-
 in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana homodimethylallyl diphosphate
0.156
-
 in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana (2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate
0.158
-
 in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana (2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate
0.234
-
 in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana (2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate