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Literature summary for 5.3.3.4 extracted from

  • Prucha, M.; Petersheim, A.; Pieper, D.H.
    Evidence for an isomeric muconolactone isomerase involved in the metabolism of 4-methylmuconolactone by Alcaligenes eutrophus JMP134 (1997), Arch. Microbiol., 168, 33-38.
    View publication on PubMed
Search for more literature for 5.3.3.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2
-
 (4R,5S)-(+)-5-Chloromuconolactone
-
 Cupriavidus necator
8.9
-
 (4S)-(+)-muconolactone
-
 Cupriavidus necator
9.8
-
 (4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10300
-
 x * 10300, SDS-PAGE Cupriavidus necator
89000
-
 gel filtration Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator
-
 basonym Alcaligenes eutrophus
-
Cupriavidus necator JMP 134-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cupriavidus necator

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:4-methylmuconolactone-grown cell
-
 Cupriavidus necator
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.09
-
-
 Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4R,5R)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5R)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator JMP 134-1 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator JMP 134-1 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5S)-(+)-5-chloromuconolactone
-
 Cupriavidus necator (2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5S)-(+)-5-chloromuconolactone
-
 Cupriavidus necator JMP 134-1 (2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5S)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator (2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5S)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator JMP 134-1 (2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
muconolactone (4S)-(+)-muconolactone Cupriavidus necator (5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
muconolactone (4S)-(+)-muconolactone Cupriavidus necator JMP 134-1 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 10300, SDS-PAGE Cupriavidus necator

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
267
-
 (4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator
317
-
 (4R,5S)-(+)-5-Chloromuconolactone
-
 Cupriavidus necator
367
-
 (4S)-(+)-muconolactone
-
 Cupriavidus necator