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Literature summary for 5.3.3.4 extracted from

  • Prucha, M.; Petersheim, A.; Timmis, K.N.; Pieper, D.H.
    Muconolactone isomerase of the 3-oxoadipate pathway catalyzes dechlorination of 5-chloro-substituted muconolactones (1996), Eur. J. Biochem., 237, 350-356.
    View publication on PubMed
Search for more literature for 5.3.3.4

Inhibitors

Inhibitors Comment Organism Structure
(4S,5S)-(+/-)-5-chloro-3-methylmuconolactone isomerization of (4R,5R)-(+/-)-5-chloro-3-methylmuconolactone Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.66
-
 (4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator
6
-
 (4S)-(+)-muconolactone
-
 Cupriavidus necator
10.3
-
 (4R,5S)-(+)-5-Chloromuconolactone
-
 Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10100
-
 10 * 10100, SDS-PAGE Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4S)-Muconolactone Cupriavidus necator
-
 ?
-
 ?
(4S)-Muconolactone Cupriavidus necator JMP 134-1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator
-
 basonym Alcaligenes eutrophus
-
Cupriavidus necator JMP 134-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cupriavidus necator

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:benzoate-grown cell
-
 Cupriavidus necator
-

Storage Stability

Storage Stability Organism
4°C, pH 7.5, 100 mM potassium sodium phosphate, 3 months stable Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4R,5R)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5R)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator JMP 134-1 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator 2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5S)-(+)-5-chloromuconolactone
-
 Cupriavidus necator (2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5S)-(+)-5-chloromuconolactone
-
 Cupriavidus necator JMP 134-1 (2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5S)-(+/-)-5-chloro-3-methylmuconolactone preferred substrate over the natural substrate (4S)-muconolactone Cupriavidus necator (2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4R,5S)-(+/-)-5-chloro-3-methylmuconolactone preferred substrate over the natural substrate (4S)-muconolactone Cupriavidus necator JMP 134-1 (2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
(4S)-Muconolactone
-
 Cupriavidus necator ?
-
 ?
(4S)-Muconolactone
-
 Cupriavidus necator JMP 134-1 ?
-
 ?
muconolactone (4S)-(+)-muconolactone Cupriavidus necator (5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
muconolactone (4S)-(+)-muconolactone Cupriavidus necator JMP 134-1 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?

Subunits

Subunits Comment Organism
decamer 10 * 10100, SDS-PAGE Cupriavidus necator

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 20 min, stable up to Cupriavidus necator
90
-
 20 min, 25% loss of activity Cupriavidus necator
100
-
-
 Cupriavidus necator

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
42.5
-
 (4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone
-
 Cupriavidus necator
683
-
 (4S)-(+)-muconolactone
-
 Cupriavidus necator
5950
-
 (4R,5S)-(+)-5-Chloromuconolactone
-
 Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 5R/4R,5S)-(+/-)-5-chloro-3-methylmuconolactone, more active in potassium sodium phosphate buffer than in Tris/HCl or borate buffer Cupriavidus necator