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Literature summary for 5.3.3.4 extracted from

  • Matsumura, E.; Sakai, M.; Hayashi, K.; Murakami, S.; Takenaka, S.; Aoki, K.
    Constitutive expression of catABC genes in the aniline-assimilating bacterium Rhodococcus species AN-22: production, purification, characterization and gene analysis of CatA, CatB and CatC (2006), Biochem. J., 393, 219-226.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.3.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.64
-
 muconolactone
-
 Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 8 * 12000, SDS-PAGE Rhodococcus sp.
100000
-
 gel filtration Rhodococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rhodococcus sp. constitutive enzyme ?
-
 ?
additional information Rhodococcus sp. AN-22 constitutive enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp. AN-22
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
860
-
-
 Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information constitutive enzyme Rhodococcus sp. ?
-
 ?
additional information constitutive enzyme Rhodococcus sp. AN-22 ?
-
 ?
muconolactone
-
 Rhodococcus sp. (5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?
muconolactone
-
 Rhodococcus sp. AN-22 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 12000, SDS-PAGE Rhodococcus sp.

Synonyms

Synonyms Comment Organism
CATC
-
 Rhodococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 pH 8.0, 10 min, enzyme maintains more than 80% of activity Rhodococcus sp.
95
-
 pH 8.0, 10 min, enzyme maintains 64% of activity Rhodococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
66.67
-
 muconolactone
-
 Rhodococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10.5 stable Rhodococcus sp.