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Literature summary for 5.3.3.4 extracted from

  • Marin, M.; Perez-Pantoja, D.; Donoso, R.; Wray, V.; Gonzalez, B.; Pieper, D.H.
    Modified 3-oxoadipate pathway for the biodegradation of methylaromatics in Pseudomonas reinekei MT1 (2010), J. Bacteriol., 192, 1543-1552.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.3.4

Cloned(Commentary)

Cloned (Comment) Organism
a 75-bp and a 125-bp fragment of the mmlJ gene are cloned into the vector pGEM-T Easy Pseudomonas reinekei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
 determined by SDS-PAGE Pseudomonas reinekei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-5-oxo-2,5-dihydrofuran-2-acetate Pseudomonas reinekei
-
 5-oxo-4,5-dihydrofuran-2-acetate
-
 ?
3-methylmuconolactone Pseudomonas reinekei
-
 4-methyl-3-oxoadipate enol-lactone
-
 ?
muconolactone Pseudomonas reinekei
-
 3-oxoadipate enol-lactone
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas reinekei C5MR75
-
-

Purification (Commentary)

Purification (Comment) Organism
MmlJ is partially purified by anion exchange chromatography using a MonoQ HR 5/5 column Pseudomonas reinekei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-5-oxo-2,5-dihydrofuran-2-acetate
-
 Pseudomonas reinekei 5-oxo-4,5-dihydrofuran-2-acetate
-
 ?
3-methylmuconolactone
-
 Pseudomonas reinekei 4-methyl-3-oxoadipate enol-lactone
-
 ?
muconolactone
-
 Pseudomonas reinekei 3-oxoadipate enol-lactone
-
 ?

Synonyms

Synonyms Comment Organism
Methylmuconolactone isomerase
-
 Pseudomonas reinekei
MmlJ
-
 Pseudomonas reinekei
Muconolactone isomerase
-
 Pseudomonas reinekei

General Information

General Information Comment Organism
metabolism the mml gene cluster consists of 10 genes encoding enzymes involved in the metabolism of 4-methylmuconolactone Pseudomonas reinekei