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Literature summary for 5.3.3.8 extracted from
- Purification and characterization of a plant peroxisomal DELTA2,DELTA3-enoyl-CoA isomerase acting on 3-cis-enoyl-CoA and 3-trans-enoyl-CoA (1991), Eur. J. Biochem., 196, 699-705.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
3-cis-hexenoyl-CoA | - |
Cucumis sativus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | exclusively localized in | Cucumis sativus | 5777 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
2 * 24000, SDS-PAGE | Cucumis sativus |
| 50000 | - |
gel filtration | Cucumis sativus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cucumis sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | fat-degrading | Cucumis sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cucumis sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-cis-Hexenoyl-CoA | most effective substrate | Cucumis sativus | ? | - |
? | |
| 3-trans-Decenoyl-CoA | 42% of the activity relative to 3-cis-hexenoyl-CoA | Cucumis sativus | 2-trans-Decenoyl-CoA | - |
? | |
| 3-trans-dodecenoyl-CoA | 89% of the activity relative to 3-cis-hexenoyl-CoA | Cucumis sativus | 2-trans-dodecenoyl-CoA | - |
? | |
| 3-trans-Hexenoyl-CoA | 2.8% of the activity relative to 3-cis-hexenoyl-CoA | Cucumis sativus | 2-trans-Hexenoyl-CoA | - |
? | |
| 3-trans-octenoyl-CoA | 40% of the activity relative to 3-cis-hexenoyl-CoA | Cucumis sativus | 2-trans-octenoyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 24000, SDS-PAGE | Cucumis sativus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Cucumis sativus |
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Release 2023.1 (January 2023)
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