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Literature summary for 5.3.3.8 extracted from

  • Zhang, D.; Yu, W.; Geisbrecht, B.V.; Gould, S.J.; Sprecher, H.; Schulz, H.
    Functional characterization of DELTA3,DELTA2-enoyl-CoA isomerases from rat liver (2002), J. Biol. Chem., 277, 9127-9132.
    View publication on PubMed
Search for more literature for 5.3.3.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0044
-
 2-trans,5-cis-octadienoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
0.0096
-
 2-trans,5-cis-tetradecadienoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
0.02
-
 2-trans,5-cis-tetradecadienoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
0.021
-
 3-cis-tetradecenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
0.028
-
 3-trans-octenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
0.029
-
 2-trans,5-cis-tetradecadienoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
0.029
-
 3-trans-tetradecenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
0.031
-
 3-cis-hexenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
0.032
-
 3-trans-tetradecenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
0.032
-
 3-cis-octenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
0.038
-
 3-trans-hexenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
0.045
-
 3-cis-tetradecenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
0.048
-
 3-trans-tetradecenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
0.057
-
 3-cis-tetradecenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
0.084
-
 2-trans,5-cis-octadienoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
0.1
-
 3-cis-octenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
0.12
-
 3-trans-octenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
0.15
-
 3-cis-octenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
0.17
-
 2-trans,5-cis-octadienoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
0.19
-
 3-trans-octenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
0.24
-
 3-cis-hexenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
0.47
-
 3-trans-hexenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
1.2
-
 3-cis-hexenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
1.6
-
 3-trans-hexenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion ECI and MECI Rattus norvegicus 5739
-
peroxisome ECI and MFE1 Rattus norvegicus 5777
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ECI, MECI and MFE1 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-trans,5-cis-octadienoyl-CoA
-
 Rattus norvegicus 3-cis,5-cis-octadienoyl-CoA
-
 ?
2-trans,5-cis-tetradecadienoyl-CoA
-
 Rattus norvegicus 3-cis,5-cis-tetradecadienoyl-CoA
-
 ?
3-cis-hexenoyl-CoA
-
 Rattus norvegicus 2-trans-hexenoyl-CoA
-
 ?
3-cis-octenoyl-CoA
-
 Rattus norvegicus 2-trans-octenoyl-CoA
-
 ?
3-cis-Tetradecenoyl-CoA
-
 Rattus norvegicus 2-trans-Tetradecenoyl-CoA
-
 ?
3-trans-Hexenoyl-CoA
-
 Rattus norvegicus 2-trans-Hexenoyl-CoA
-
 ?
3-trans-octenoyl-CoA
-
 Rattus norvegicus 2-trans-octenoyl-CoA
-
 ?
3-trans-tetradecenoyl-CoA
-
 Rattus norvegicus 2-trans-tetradecenoyl-CoA
-
 ?
additional information MECI is most active in catalyzing the 3-cis/2-trans isomerization, ECI has a preference for the 3-trans/2-trans isomerization, and MFE1 is the optimal isomerase for the 2,5-/3,5-isomerization. 3-cis/2-trans and 2,5/3,5 isomerization in mitochondria are catalyzed overwhelmingly by MECI, whereas ECI contributes significantly to the 3-trans/2-trans isomerization. In peroxisomes, ECI is predicted to be the dominant enzyme for the 3-cis/2-trans and 3-trans/2-trans isomerization of long chain intermediates Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
ECI monofunctional enoyl CoA isomerase Rattus norvegicus
MECI mitochondrial enoyl-CoA isomerase, present only in the mitochondria Rattus norvegicus
MFE1 multifunctional enzyme 1 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 3-trans-tetradecenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
1.6
-
 3-cis-hexenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
1.8
-
 3-trans-hexenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
2
-
 3-trans-octenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
2.4
-
 3-cis-octenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
2.7
-
 3-cis-tetradecenoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
8.3
-
 2-trans,5-cis-tetradecadienoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
11
-
 2-trans,5-cis-octadienoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
12
-
 2-trans,5-cis-tetradecadienoyl-CoA pH 8.0, 25°C, MFE1 Rattus norvegicus
15
-
 2-trans,5-cis-tetradecadienoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
20
-
 3-trans-tetradecenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
25
-
 3-trans-octenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
26
-
 2-trans,5-cis-octadienoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
45
-
 2-trans,5-cis-octadienoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
47
-
 3-cis-octenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
58
-
 3-cis-hexenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
98
-
 3-cis-tetradecenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
100
-
 3-trans-hexenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
180
-
 3-cis-octenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
200
-
 3-cis-hexenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
210
-
 3-cis-tetradecenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
210
-
 3-trans-octenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus
270
-
 3-trans-hexenoyl-CoA pH 8.0, 25°C, MECI Rattus norvegicus
540
-
 3-trans-tetradecenoyl-CoA pH 8.0, 25°C, ECI Rattus norvegicus