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Literature summary for 5.3.99.4 extracted from

  • Chao, W.C.; Lu, J.F.; Wang, J.S.; Yang, H.C.; Chen, H.H.; Lan, Y.K.; Yu, Y.C.; Chou, P.T.; Wang, L.H.
    Probing the interaction between prostacyclin synthase and prostaglandin H2 analogues or inhibitors via a combination of resonance Raman spectroscopy and molecular dynamics simulation approaches (2011), J. Am. Chem. Soc., 133, 18870-18879.
    View publication on PubMed
Search for more literature for 5.3.99.4

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally His4-tagged enzyme with the first 16 amino acid residues in the N-terminal transmembrane domain replaced with a hydrophilic sequence, MAKKTSS, in Escherichia coli strain BL21/(DE3)pLys Danio rerio
recombinant expression of C-terminally His4-tagged enzyme with the first 17 amino acid residues in the N-terminal transmembrane domain replaced with a hydrophilic sequence, MAKKTSS, in Escherichia coli strain BL21/(DE3)pLys Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
clotrimazole 1-(o-chloro-alpha,alpha-diphenylbenzyl)imidazole Danio rerio
clotrimazole 1-(o-chloro-alpha,alpha-diphenylbenzyl)imidazole Homo sapiens
miconazole i.e. 1-[2,4-dichloro-beta-[(2,4-dichlorobenzyl)oxy]phenyl]imidazole Danio rerio
miconazole i.e. 1-[2,4-dichloro-beta-[(2,4-dichlorobenzyl)oxy]phenyl]imidazole Homo sapiens
minoxidil 6-(1-piperidyl)-2,4-diaminopyrimidine 3-oxide Danio rerio
minoxidil 6-(1-piperidyl)-2,4-diaminopyrimidine 3-oxide Homo sapiens

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His4-tagged modified enzyme from Escherichia coli strain BL21/(DE3)pLys by nickel affinity chromatography and carboxymethyl cation exchange chromatography to homogeneity Homo sapiens
recombinant C-terminally His4-tagged modified enzyme from Escherichia coli strain BL21/(DE3)pLys by nickel affinity chromatography and carboxymethyl cation exchange chromatography to homogeneity Danio rerio

Synonyms

Synonyms Comment Organism
PGIS
-
 Homo sapiens
PGIS
-
 Danio rerio
prostacyclin synthase
-
 Homo sapiens
prostacyclin synthase
-
 Danio rerio

Cofactor

Cofactor Comment Organism Structure
heme heme conformation and heme protein matrix interactions for human and zebrafish enzymes in the presence and absence of ligands, overview. The heme group is in the ferric, six-coordinate, low-spin state for both resting and ligand-bound enzymes of boh species. Drastically different RR shifts with heme conformational changes in both hPGIS and zPGIS upon different ligand bindings, suggesting that PGIS exhibits a ligand-specific heme conformational change to accommodate the substrate binding Homo sapiens
heme heme conformation and heme protein matrix interactions for human and zebrafish enzymes in the presence and absence of ligands, overview. The heme group is in the ferric, six-coordinate, low-spin state for both resting and ligand-bound enzymes of boh species. Drastically different RR shifts with heme conformational changes in both hPGIS and zPGIS upon different ligand bindings, suggesting that PGIS exhibits a ligand-specific heme conformational change to accommodate the substrate binding Danio rerio

General Information

General Information Comment Organism
additional information structure-function relationship analysis by a combination of resonance Raman spectroscopy and molecular dynamics simulation approaches using prostaglandin H2 analogues U46619 or (Z)-7-[(1S,4R,5R,6S)-5-[(E,3S)-3-hydroxyoct-1-enyl]-3-oxabicyclo[2.2.1]heptan-6-yl]hept-5-enoic acid, U51605 or 9alpha,11alpha-azoprosta-5Z,13E-dien-1-oic acid, and U44069 or (Z)-7-[(1R,4S,5R,6S)-6-[(E,3S)-3-hydroxyoct-1-enyl]-3-oxabicyclo[2.2.1]heptan-5-yl]hept-5-enoic acid and inhibitors minoxidil, clotrimazole, miconazole, overview Homo sapiens
additional information structure-function relationship analysis by a combination of resonance Raman spectroscopy and molecular dynamics simulation approaches using prostaglandin H2 analogues U46619 or (Z)-7-[(1S,4R,5R,6S)-5-[(E,3S)-3-hydroxyoct-1-enyl]-3-oxabicyclo[2.2.1]heptan-6-yl]hept-5-enoic acid, U51605 or 9alpha,11alpha-azoprosta-5Z,13E-dien-1-oic acid, and U44069 or (Z)-7-[(1R,4S,5R,6S)-6-[(E,3S)-3-hydroxyoct-1-enyl]-3-oxabicyclo[2.2.1]heptan-5-yl]hept-5-enoic acid and inhibitors minoxidil, clotrimazole, miconazole, overview Danio rerio