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Literature summary for 5.4.2.2 extracted from

  • Popova, T.N.; Matasova, L.V.; Lapot'ko, A.A.
    Purification, separation and characterization of phosphoglucomutase and phosphomannomutase from maize leaves (1998), Biochem. Mol. Biol. Int., 46, 461-470.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-glucose-1,6-bisphosphate
-
Zea mays

Inhibitors

Inhibitors Comment Organism Structure
D-Glucose 1,6-bisphosphate
-
Zea mays
D-glucose 1-phosphate
-
Zea mays
D-mannose 1-phosphate
-
Zea mays
Mn2+ approx. 50% inhibition at 5 mM, almost complete inhibition at 7 mM Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
alpha-D-Glucose 1,6-bisphosphate 25°C, pH 7.6 Zea mays
0.02
-
alpha-D-glucose 1-phosphate 25°C, pH 7.6 Zea mays
0.02
-
D-mannose 1-phosphate 25°C, pH 7.6 Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ approx. 4fold activation at 4 mM Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
2 * 66000, SDS-PAGE Zea mays
132000
-
gel filtration Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucose 1-phosphate Zea mays
-
alpha-D-glucose 6-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-25, DEAE-Toyopearl, Toyopearl HW-65 Zea mays

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.7
-
-
Zea mays

Storage Stability

Storage Stability Organism
0-4°C, 50% glycerol, 2-3 d, no loss of activity Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1,6-bisphosphate + H2O
-
Zea mays alpha-D-glucose 6-phosphate + phosphate
-
?
alpha-D-glucose 1-phosphate
-
Zea mays alpha-D-glucose 6-phosphate
-
r
alpha-D-glucose 1-phosphate
-
Zea mays D-glucose 6-phosphate
-
?
alpha-D-mannose 1-phosphate
-
Zea mays alpha-D-mannose 6-phosphate
-
r
D-Mannose 1-phosphate
-
Zea mays D-Mannose 6-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 66000, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
PGM
-
Zea mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
no loss of activity after 3 min Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Zea mays

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.23
-
D-Glucose 1,6-bisphosphate 25°C, pH 7.6, substrate inhibition at high concentrations Zea mays
1.35
-
D-mannose 1-phosphate 25°C, pH 7.6, substrate inhibition at high concentrations Zea mays
3.3
-
D-glucose 1-phosphate 25°C, pH 7.6, substrate inhibition at high concentrations Zea mays