Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucose-1,6-bisphosphate | - |
Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Glucose 1,6-bisphosphate | - |
Zea mays | |
D-glucose 1-phosphate | - |
Zea mays | |
D-mannose 1-phosphate | - |
Zea mays | |
Mn2+ | approx. 50% inhibition at 5 mM, almost complete inhibition at 7 mM | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
alpha-D-Glucose 1,6-bisphosphate | 25°C, pH 7.6 | Zea mays | |
0.02 | - |
alpha-D-glucose 1-phosphate | 25°C, pH 7.6 | Zea mays | |
0.02 | - |
D-mannose 1-phosphate | 25°C, pH 7.6 | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | approx. 4fold activation at 4 mM | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
2 * 66000, SDS-PAGE | Zea mays |
132000 | - |
gel filtration | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | Zea mays | - |
alpha-D-glucose 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephadex G-25, DEAE-Toyopearl, Toyopearl HW-65 | Zea mays |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.7 | - |
- |
Zea mays |
Storage Stability | Organism |
---|---|
0-4°C, 50% glycerol, 2-3 d, no loss of activity | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1,6-bisphosphate + H2O | - |
Zea mays | alpha-D-glucose 6-phosphate + phosphate | - |
? | |
alpha-D-glucose 1-phosphate | - |
Zea mays | alpha-D-glucose 6-phosphate | - |
r | |
alpha-D-glucose 1-phosphate | - |
Zea mays | D-glucose 6-phosphate | - |
? | |
alpha-D-mannose 1-phosphate | - |
Zea mays | alpha-D-mannose 6-phosphate | - |
r | |
D-Mannose 1-phosphate | - |
Zea mays | D-Mannose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 66000, SDS-PAGE | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
PGM | - |
Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
no loss of activity after 3 min | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Zea mays |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
D-Glucose 1,6-bisphosphate | 25°C, pH 7.6, substrate inhibition at high concentrations | Zea mays | |
1.35 | - |
D-mannose 1-phosphate | 25°C, pH 7.6, substrate inhibition at high concentrations | Zea mays | |
3.3 | - |
D-glucose 1-phosphate | 25°C, pH 7.6, substrate inhibition at high concentrations | Zea mays |