Literature summary for 5.4.2.2 extracted from

Mehra-Chaudhary, R.; Mick, J.; Tanner, J.J.; Henzl, M.T.; Beamer, L.J.
Crystal structure of a bacterial phosphoglucomutase, an enzyme involved in the virulence of multiple human pathogens (2011), Proteins, 79, 1215-1229.

Search for more literature for 5.4.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 0.1 M Bis-Tris, pH 6.5, 0.2 M MgCl2, and 25% (w/v) PEG 3350	Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	a single Mg2+ ion is required for catalytic activity, each PGM monomer contains one bound Mg2+ ion in its metal binding site	Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60800	-	2 * 60800, calculated from amino acid sequence	Salmonella enterica subsp. enterica serovar Typhimurium
105200	-	dynamic light scattering	Salmonella enterica subsp. enterica serovar Typhimurium
109600	-	analytical ultracentrifugation	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	Q8ZQW9	serovar typhimurium	-

Purification (Commentary)

Purification (Comment)	Organism
His-Select Ni-Affinity gel column chromatography, gel filtration	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-glucose 1-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	alpha-D-glucose 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 60800, calculated from amino acid sequence	Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms	Comment	Organism
PGM	-	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)