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Literature summary for 5.4.2.2 extracted from

  • Stiers, K.M.; Graham, A.C.; Kain, B.N.; Beamer, L.J.
    Asp263 missense variants perturb the active site of human phosphoglucomutase 1 (2017), FEBS J., 284, 937-947 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
alpha-D-Glucose 1,6-bisphosphate dependent on Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, mutant enzyme D263Y crystals grow in solutions including lithium or ammonium sulfate (1.4-1.55 M) with 0.1 M buffer of either MES, pH 6.0, or Tris HCl, pH 7.5. Crystals of mutant enzyme D263G grow from 1.6 M ammonium sulfate, 0.1 M NaCl, and 0.1 M HEPES, pH 7.5 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D263G the mutation is associated with phosphoglucomutase 1 deficiency Homo sapiens
D263Y the mutation is associated with phosphoglucomutase 1 deficiency Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucose 1-phosphate Homo sapiens
-
D-glucose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P36871
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate
-
Homo sapiens D-glucose 6-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 65000, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
PGM1
-
Homo sapiens
phosphoglucomutase 1
-
Homo sapiens