Literature summary for 5.4.2.8 extracted from

Pirard, M.; Achouri, Y.; Collet, J.F.; Schollen, E.; Matthijs, G.; Van Schaftingen, E.
Kinetic properties and tissular distribution of mammalian phosphomannomutase isozymes (1999), Biochem. J., 339, 201-207.

No PubMed abstract available

Search for more literature for 5.4.2.8

Activating Compound

Activating Compound	Comment	Organism	Structure
D-Glucose 1,6-bisphosphate	PMM2 requires a hexose bisphosphate for activity, as potent as mannose 1,6-bisphosphate in activation	Homo sapiens
D-Mannose 1,6-bisphosphate	PMM2 requires a hexose bisphosphate for activity, as potent as glucose 1,6-bisphosphate in activation, half-maximal activation in the presence of 0.01 mM and 0.1 mM at 0.0005 mM and 0.001 mM mannose 1,6-bisphosphate, respectively	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
vanadate	0.05 mM, 50% inhibition	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.012	-	alpha-D-glucose 1-phosphate	-	Homo sapiens
0.018	-	D-mannose 1-phosphate	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
alpha-D-mannose 1-phosphate	Homo sapiens	-	alpha-D-mannose 6-phosphate	-	r
alpha-D-mannose 1-phosphate	Rattus norvegicus	20fold higher activity than with alpha-D-glucose 1-phosphate	alpha-D-mannose 6-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant PMM2, polyethylene glycol, DEAE-Sepharose, Q-Sepharose	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
49	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-glucose 1-phosphate	-	Homo sapiens	alpha-D-glucose 6-phosphate	-	r
alpha-D-glucose 1-phosphate	-	Homo sapiens	D-glucose 6-phosphate	-	?
alpha-D-mannose 1-phosphate	-	Homo sapiens	alpha-D-mannose 6-phosphate	-	r
alpha-D-mannose 1-phosphate	20fold higher activity than with alpha-D-glucose 1-phosphate	Homo sapiens	alpha-D-mannose 6-phosphate	-	r
alpha-D-mannose 1-phosphate	20fold higher activity than with alpha-D-glucose 1-phosphate	Rattus norvegicus	alpha-D-mannose 6-phosphate	-	r

Synonyms

Synonyms	Comment	Organism
PMM2	-	Homo sapiens
PMM2	-	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.1	-	80% of maximal activity	Homo sapiens

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Release 2023.1 (January 2023)