Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2'-deoxyATP | or ATP required | Acetoanaerobium sticklandii | |
2-oxobutanoate | can partially replace pyruvate, which is required for reaction | Acetoanaerobium sticklandii | |
mercaptan | required | Acetoanaerobium sticklandii | |
pyruvate | required | Acetoanaerobium sticklandii |
General Stability | Organism |
---|---|
highly purified sulfhydryl-enzyme-component is relatively unstable in dilute solutions of low ionic strength. The presence of mercaptans in buffers tends to prevent aggregation. The cobamide enzyme component is stable to a variety of storage conditions even when highly purified | Acetoanaerobium sticklandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Acetoanaerobium sticklandii | |
Co2+ | - |
Acetoanaerobium sticklandii | |
iodoacetamide | cobamide enzyme compound is unaffected, sulfhydryl enzyme compounds is rapidly inactivated | Acetoanaerobium sticklandii | |
NH4+ | - |
Acetoanaerobium sticklandii | |
Tris | and analogs | Acetoanaerobium sticklandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulates | Acetoanaerobium sticklandii | |
Mg2+ | Mg2+ or Mn2+ required | Acetoanaerobium sticklandii | |
Mn2+ | Mg2+ or Mn2+ required | Acetoanaerobium sticklandii | |
Rb+ | stimulates | Acetoanaerobium sticklandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, gel filtration, sulfhydryl component, x * about approximately 150000, gel filtration, cobamide protein component | Acetoanaerobium sticklandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,6-Diaminohexanoate | r | Acetoanaerobium sticklandii | 3,5-Diaminohexanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, gel filtration, sulfhydryl component, x * about approximately 150000, gel filtration, cobamide protein component | Acetoanaerobium sticklandii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, sulfhydryl component is destroyed, cobamide protein component loses about 40% of its activity | Acetoanaerobium sticklandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Acetoanaerobium sticklandii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
alpha-(adenyl)-Co-5'-deoxyadenosyl cobamide | tightly bound | Acetoanaerobium sticklandii | |
ATP | or 2'-deoxyATP required | Acetoanaerobium sticklandii | |
FAD | stimulates | Acetoanaerobium sticklandii |