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Literature summary for 5.4.3.3 extracted from

  • Maity, A.; Lin, H.; Chiang, H.; Lo, H.; Ke, S.
    Reaction of pyridoxal-5'-phosphate-N-oxide with lysine 5,6-aminomutase Enzyme flexibility toward cofactor analog (2015), ACS Catal., 5, 3093-3099 .
No PubMed abstract available
Search for more literature for 5.4.3.3

Cloned(Commentary)

Cloned (Comment) Organism
genes kamD and kam E encode subunits alpha and beta Acetoanaerobium sticklandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters of enzyme 5,6-LAM are measured using D-lysine or isotope-labeled D-lysine-4,4,5,5-d4 as a substrate, overview Acetoanaerobium sticklandii
1
-
 D-Lysine with pyridoxal 5'-phosphate N-oxide as cofactor, pH 8.5, 37°C Acetoanaerobium sticklandii
14.2
-
 D-Lysine with pyridoxal 5'-phosphate as cofactor, pH 8.5, 37°C Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S)-3,6-diaminohexanoate Acetoanaerobium sticklandii
-
 (3S,5S)-3,5-diaminohexanoate
-
 r

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii E3PRJ5 AND E3PRJ4 alpha- and beta-subunits
-

Reaction

Reaction Comment Organism Reaction ID
(3S)-3,6-diaminohexanoate = (3S,5S)-3,5-diaminohexanoate reaction mechanism, detailed overview Acetoanaerobium sticklandii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-3,6-diaminohexanoate
-
 Acetoanaerobium sticklandii (3S,5S)-3,5-diaminohexanoate
-
 r
D-lysine
-
 Acetoanaerobium sticklandii 2,5-diaminohexanoate
-
 r

Synonyms

Synonyms Comment Organism
5,6-LAM
-
 Acetoanaerobium sticklandii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Acetoanaerobium sticklandii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 D-Lysine with pyridoxal 5'-phosphate N-oxide as cofactor, pH 8.5, 37°C Acetoanaerobium sticklandii
14.2
-
 D-Lysine with pyridoxal 5'-phosphate as cofactor, pH 8.5, 37°C Acetoanaerobium sticklandii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Acetoanaerobium sticklandii

Cofactor

Cofactor Comment Organism Structure
5'-deoxyadenosylcobalamin
-
 Acetoanaerobium sticklandii
additional information lysine 5,6-aminomutase is a 5'-deoxyadenosylcobalamin and pyridoxal 5'-phosphate co-dependent radical enzyme, formation of internal aldimine with pyridoxal 5'-phosphate and pyridoxal 5'-phosphate-N-oxide Acetoanaerobium sticklandii
additional information lysine 5,6-aminomutase shows flexibility toward cofactor analogue pyridoxal 5'-phosphate-N-oxide, which can act as a functional cofactor for enzyme 5,6-LAM, kinetics and structure-function analysis, overview Acetoanaerobium sticklandii
pyridoxal 5'-phosphate
-
 Acetoanaerobium sticklandii