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Literature summary for 5.4.3.8 extracted from

  • Tsang, E.W.; Hu, Z.; Chang, Q.; McGregor, D.I.; Keller, W.A.
    Expression of a Brassic napus glutamate 1-semialdehyde aminotransferase in Escherichia coli and characterization of the recombinant protein (2003), Protein Expr. Purif., 29, 193-201.
    View publication on PubMed
Search for more literature for 5.4.3.8

Activating Compound

Activating Compound Comment Organism Structure
glutamate 3fold activation of tagless recombinant enzyme Brassica napus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Brassica napus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
 2 * 45500, SDS-PAGE, native and recombinant GSA-AT Brassica napus
88000
-
 native and recombinant GSA-AT, gel filtration Brassica napus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-4-amino-5-oxopentanoate Brassica napus involved in chlorophyll biosynthesis 5-aminolevulinate
-
 r

Organism

Organism UniProt Comment Textmining
Brassica napus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GSA-AT Brassica napus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
 recombinant GSA-AT Brassica napus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-4-amino-5-oxopentanoate
-
 Brassica napus 5-aminolevulinate
-
 r
(S)-4-amino-5-oxopentanoate involved in chlorophyll biosynthesis Brassica napus 5-aminolevulinate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 45500, SDS-PAGE, native and recombinant GSA-AT Brassica napus

Synonyms

Synonyms Comment Organism
GSA-AT
-
 Brassica napus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Brassica napus