Literature summary for 5.4.3.8 extracted from

Ge, H.; Lv, X.; Fan, J.; Gao, Y.; Teng, M.; Niu, L.
Crystal structure of glutamate-1-semialdehyde aminotransferase from Bacillus subtilis with bound pyridoxamine-5-phosphate (2010), Biochem. Biophys. Res. Commun., 402, 356-360.

Search for more literature for 5.4.3.8

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with pyridoxamine 5'-phosphate, hanging drop vapor diffusion method, using 0.1 M Bicine pH 8.5, 30% (w/v) PEG 3350, at 23°C	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P30949	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-chelating resin column chromatography and Superdex 200 gel filtration	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate 1-semialdehyde	-	Bacillus subtilis	5-aminolevulinate	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
glutamate-1-semialdehyde aminomutase	-	Bacillus subtilis
Glutamate-1-semialdehyde aminotransferase	-	Bacillus subtilis
GSA-AT	-	Bacillus subtilis
GSAM	-	Bacillus subtilis

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	dependent on	Bacillus subtilis
pyridoxamine 5'-phosphate	dependent on	Bacillus subtilis

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Release 2023.1 (January 2023)