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Literature summary for 5.4.3.8 extracted from

  • Ramzi, A.B.; Hyeon, J.E.; Kim, S.W.; Park, C.; Han, S.O.
    5-Aminolevulinic acid production in engineered Corynebacterium glutamicum via C5 biosynthesis pathway (2015), Enzyme Microb. Technol., 81, 1-7 .
    View publication on PubMed
Search for more literature for 5.4.3.8

Cloned(Commentary)

Cloned (Comment) Organism
gene hemL from Corynebacterium glutamicum, recombinant co-expression with gene hemA, encoding the glutamyl t-RNA reductase, from Salmonella typhimurium strain LT2, in Corynebacterium glutamicum strain ATCC 13032 in an operon under the control of trc promoter, and transcription is terminated with the rrnBT1T2 terminator, subcloning of hemL in Escherichia coli strain BL21(DE3) Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
additional information improved biological production of 5-aminolevulinate in Corynebacterium glutamicum is achieved by overexpressing the glutamate-initiated C5 pathway. Copies of the glutamyl t-RNA reductase HemA from several bacteria (Corynebacterium glutamicum, Escherichia coli, Bacillus subtilis, Salmonella typhimurium, and Klebsiella pneumoniae) are mutated by site-directed mutagenesis of which a HemA version from Salmonella typhimurium exhibits the highest 5-aminolevulinate production. Cultivation of the HemA-expressing strain produces approximately 204 mg/l of 5-aminolevulinate, while co-expression with HemL (glutamate-1-semialdehyde amino-transferase) increases 5-aminolevulinate concentration to 457 mg/L, representing 11.6fold and 25.9fold increases over the control strain (17 mg/l of 5-aminolevulinate). 5-Aminolevulinate overproduction can also be increased by reducing the formation of heme, which has been shown to have inhibitory effects on HemA activity. Method evaluation and optimization, overview Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate 1-semialdehyde Corynebacterium glutamicum
-
 5-aminolevulinate
-
 ?
L-glutamate 1-semialdehyde Corynebacterium glutamicum ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025
-
 5-aminolevulinate
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum Q8NT73
-
-
Corynebacterium glutamicum ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025 Q8NT73
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate 1-semialdehyde
-
 Corynebacterium glutamicum 5-aminolevulinate
-
 ?
L-glutamate 1-semialdehyde
-
 Corynebacterium glutamicum ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025 5-aminolevulinate
-
 ?

Synonyms

Synonyms Comment Organism
glutamate-1-semialdehyde amino-transferase
-
 Corynebacterium glutamicum
HemL
-
 Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Corynebacterium glutamicum