Application | Comment | Organism |
---|---|---|
synthesis | trehalose production from starch | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
maltoheptaose | pH 5.5, 60°C | Saccharolobus solfataricus | |
2.7 | - |
maltohexaose | pH 5.5, 60°C | Saccharolobus solfataricus | |
5 | - |
maltopentaose | pH 5.5, 60°C | Saccharolobus solfataricus | |
21.8 | - |
maltotetraose | pH 5.5, 60°C | Saccharolobus solfataricus | |
228 | - |
maltotriose | pH 5.5, 60°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
x * 76000, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus KM1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltoheptaose | - |
Saccharolobus solfataricus | alpha-maltopentaosyl trehalose | - |
? | |
maltoheptaose | - |
Saccharolobus solfataricus KM1 | alpha-maltopentaosyl trehalose | - |
? | |
maltohexaose | - |
Saccharolobus solfataricus | alpha-maltotetraosyltrehalose | - |
? | |
maltohexaose | - |
Saccharolobus solfataricus KM1 | alpha-maltotetraosyltrehalose | - |
? | |
maltopentaose | - |
Saccharolobus solfataricus | alpha-maltotriosyltrehalose | - |
? | |
maltopentaose | - |
Saccharolobus solfataricus KM1 | alpha-maltotriosyltrehalose | - |
? | |
maltotetraose | - |
Saccharolobus solfataricus | alpha-maltosyltrehalose | - |
? | |
maltotetraose | - |
Saccharolobus solfataricus KM1 | alpha-maltosyltrehalose | - |
? | |
maltotriose | - |
Saccharolobus solfataricus | alpha-glucosyltrehalose | - |
? | |
additional information | no activity with maltose. The enzyme never produces a glycosyltrehalose of longer chain length than the substrate used. Glucose and maltooligosaccharide shortened by one glucose residue are produced as by-products | Saccharolobus solfataricus | ? | - |
? | |
additional information | no activity with maltose. The enzyme never produces a glycosyltrehalose of longer chain length than the substrate used. Glucose and maltooligosaccharide shortened by one glucose residue are produced as by-products | Saccharolobus solfataricus KM1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
6 h, 9% loss of activity | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | stable | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | isoelectric focusing | - |
6.1 |