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Literature summary for 5.4.99.17 extracted from

  • Morikubo, N.; Fukuda, Y.; Ohtake, K.; Shinya, N.; Kiga, D.; Sakamoto, K.; Asanuma, M.; Hirota, H.; Yokoyama, S.; Hoshino, T.
    Cation-pi interaction in the polyolefin cyclization cascade uncovered by incorporating unnatural amino acids into the catalytic sites of squalene cyclase (2006), J. Am. Chem. Soc., 128, 13184-13194.
    View publication on PubMed
Search for more literature for 5.4.99.17

Protein Variants

Protein Variants Comment Organism
F365W marginal catalytic activity Alicyclobacillus acidocaldarius
F365Y and mutant with F365 changed to unnatural amino acid O-methyltyrosine. Both show increased decreased activity at high temperature Alicyclobacillus acidocaldarius
F605W increased catalytic acitivy at low temperature, but decreased activity at high temperature due to higher cation-pi binding energies Alicyclobacillus acidocaldarius
F605Y and mutant with F605 changed to unnatural amino acid O-methyltyrosine. Both show increased catalytic acitivy at low temperature, but decreased activity at high temperature due to higher cation-pi binding energies Alicyclobacillus acidocaldarius
additional information replacement of F605 by mono-, di- or trifluorophenylalanine, with or without additional mutation Y606A, kinetic analysis. Mutant F605 changed to trifluorophenylalanine plus mutation Y606A has negligibly small activity Alicyclobacillus acidocaldarius
Y606A kinetics identical to wild-type Alicyclobacillus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0154
-
 squalene wild-type, pH 6.0, 40°C Alicyclobacillus acidocaldarius
0.0242
-
 squalene wild-type, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.0279
-
 squalene mutant F605 changed to fluorophenylalanine plus mutation Y606A, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.0324
-
 squalene mutant F605 changed to difluorophenylalanine plus mutation Y606A, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.0352
-
 squalene mutant F365 changed to fluorophenylalanine, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.0416
-
 squalene mutant F365 changed to difluorophenylalanine, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.0479
-
 squalene mutant F6365 changed to trifluorophenylalanine, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.0525
-
 squalene mutant F605Y, pH 6.0, 40°C Alicyclobacillus acidocaldarius
0.104
-
 squalene mutant F605Y, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.105
-
 squalene mutant F605 changed to O-methyltyrosine, pH 6.0, 40°C Alicyclobacillus acidocaldarius
0.118
-
 squalene mutant F605W, pH 6.0, 40°C Alicyclobacillus acidocaldarius
0.13
-
 squalene mutant F365Y, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.152
-
 squalene mutant F605Y changed to O-methyltyrosine, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.185
-
 squalene mutant F365Y changed to O-methyltyrosine, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.191
-
 squalene mutant F605W, pH 6.0, 50°C Alicyclobacillus acidocaldarius

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
squalene
-
 Alicyclobacillus acidocaldarius hop-22(29)-ene
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 wild-type Alicyclobacillus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 squalene mutant F365Y changed to O-methyltyrosine, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.17
-
 squalene mutant F365Y, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.25
-
 squalene wild-type, pH 6.0, 40°C Alicyclobacillus acidocaldarius
0.27
-
 squalene mutant F605Y, pH 6.0, 40°C Alicyclobacillus acidocaldarius
0.31
-
 squalene mutant F605W, pH 6.0, 40°C Alicyclobacillus acidocaldarius
0.45
-
 squalene mutant F605 changed to O-methyltyrosine, pH 6.0, 40°C Alicyclobacillus acidocaldarius
0.54
-
 squalene mutant F605 changed to difluorophenylalanine plus mutation Y606A, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.7
-
 squalene mutant F605Y changed to O-methyltyrosine, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.75
-
 squalene mutant F6365 changed to trifluorophenylalanine, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.82
-
 squalene mutant F605 changed to fluorophenylalanine plus mutation Y606A, pH 6.0, 50°C Alicyclobacillus acidocaldarius
0.92
-
 squalene mutant F365 changed to difluorophenylalanine, pH 6.0, 50°C Alicyclobacillus acidocaldarius
1.1
-
 squalene mutant F365 changed to fluorophenylalanine, pH 6.0, 50°C Alicyclobacillus acidocaldarius
1.32
-
 squalene mutant F605W, pH 6.0, 50°C Alicyclobacillus acidocaldarius
1.82
-
 squalene mutant F605Y, pH 6.0, 50°C Alicyclobacillus acidocaldarius
1.98
-
 squalene wild-type, pH 6.0, 50°C Alicyclobacillus acidocaldarius