Protein Variants | Comment | Organism |
---|---|---|
A419F | inactive | Alicyclobacillus acidocaldarius |
D376C | inactive | Alicyclobacillus acidocaldarius |
F365A | the mutant shows increased activity with geraniol and (S)-6,7-epoxygeraniol compared to the wild type enzyme | Alicyclobacillus acidocaldarius |
F365C | the mutant exhibits 15% conversion of geraniol to gamma-cyclogeraniol | Alicyclobacillus acidocaldarius |
F365C | the mutant shows increased activity with geraniol compared to the wild type enzyme | Alicyclobacillus acidocaldarius |
F365G | the mutant shows increased activity with (S)-6,7-epoxygeraniol and no activity with geraniol compared to the wild type enzyme | Alicyclobacillus acidocaldarius |
F365W | inactive | Alicyclobacillus acidocaldarius |
F601A | the mutant shows about wild type activity | Alicyclobacillus acidocaldarius |
F601W | the mutant shows about wild type activity | Alicyclobacillus acidocaldarius |
G600F | the mutant exhibits 68% conversion of geraniol to cyclogeraniol hydrate | Alicyclobacillus acidocaldarius |
I261A | the mutant exhibits 11% conversion of citronellal to (-)-iso-isopulegol. The mutant shows increased activity with geraniol and (S)-6,7-epoxygeraniol compared to the wild type enzyme | Alicyclobacillus acidocaldarius |
I261W | the mutant shows increased activity with (S)-6,7-epoxygeraniol and no activity with geraniol compared to the wild type enzyme | Alicyclobacillus acidocaldarius |
L36A | the mutant shows increased activity with (S)-6,7-epoxygeraniol compared to the wild type enzyme | Alicyclobacillus acidocaldarius |
L36W | inactive | Alicyclobacillus acidocaldarius |
S307A | the mutant shows increased activity with (S)-6,7-epoxygeraniol and no activity with geraniol compared to the wild type enzyme | Alicyclobacillus acidocaldarius |
S307F | inactive | Alicyclobacillus acidocaldarius |
V448F | inactive | Alicyclobacillus acidocaldarius |
W169A | the mutant shows about wild type activity | Alicyclobacillus acidocaldarius |
W312A | inactive | Alicyclobacillus acidocaldarius |
W489A | inactive | Alicyclobacillus acidocaldarius |
Y420A | inactive | Alicyclobacillus acidocaldarius |
Y420C | the mutant shows about wild type activity | Alicyclobacillus acidocaldarius |
Y420W | the mutant exhibits 54% conversion of (S)-6,7-epoxygeraniol to (1S,3R)-3-(hydroxymethyl)-2,2-dimethyl-4-methylidenecyclohexan-1-ol | Alicyclobacillus acidocaldarius |
Y420W/G600F | the mutant exhibits 78% conversion of (S)-6,7-epoxygeraniol to (1S,3R)-3-(hydroxymethyl)-2,2-dimethyl-4-methylidenecyclohexan-1-ol | Alicyclobacillus acidocaldarius |
Y609W | inactive | Alicyclobacillus acidocaldarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
squalene | Alicyclobacillus acidocaldarius | - |
hop-22(29)-ene | - |
? | |
squalene + H2O | Alicyclobacillus acidocaldarius | - |
hopan-22-ol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus acidocaldarius | P33247 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-6,7-epoxygeraniol + H2O | 0.57% conversion with high enzyme concentrations | Alicyclobacillus acidocaldarius | (1S,3R)-3-(hydroxymethyl)-2,2-dimethyl-4-methylidenecyclohexan-1-ol | - |
? | |
(S)-citronellal + H2O | 0.59% conversion with high enzyme concentrations | Alicyclobacillus acidocaldarius | (-)-iso-isopulegol | - |
? | |
geraniol + H2O | 0.4% conversion with high enzyme concentrations | Alicyclobacillus acidocaldarius | gamma-cyclogeraniol + cyclogeraniol hydrate | - |
? | |
squalene | - |
Alicyclobacillus acidocaldarius | hop-22(29)-ene | - |
? | |
squalene + H2O | - |
Alicyclobacillus acidocaldarius | hopan-22-ol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SHC | - |
Alicyclobacillus acidocaldarius |
squalene hopene cyclase | - |
Alicyclobacillus acidocaldarius |