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Literature summary for 5.4.99.25 extracted from

  • Phannachet, K.; Elias, Y.; Huang, R.H.
    Dissecting the roles of a strictly conserved tyrosine in substrate recognition and catalysis by pseudouridine 55 synthase (2005), Biochemistry, 44, 15488-15494.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the Y67F mutant in complex with a 5-fluorouridine-tRNA at 2.4 A resolution Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
Y67F no activity with the natural RNA substrate, efficient formation of 5-fluoro-6-hydroxypseudouridine55 from 5-fluorouridine55 Thermotoga maritima
Y67L no activity with the natural RNA substrate, efficient formation of 5-fluoro-6-hydroxypseudouridine55 from 5-fluorouridine55 Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00087
-
tRNA uridine55 wild-type enzyme Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WZW0
-
-

Reaction

Reaction Comment Organism Reaction ID
tRNA uridine55 = tRNA pseudouridine55 the side chain of the conserved tyrosine in the four families of pseudouridine synthases (TruA, TruB, RsuA and RluA) plays a dual role within the active site, maintaining the structural integrity of the active site through its hydrophobic phenyl ring and acting as a general base through its OH group for the proton abstraction required in the last step of pseudouridine synthase-catalyzed formation of pseudouridine Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information efficient formation of 5-fluoro-6-hydroxypseudouridine55 from 5-fluorouridine55 is catalyzed by wild-type enzyme and mutant enzymes Y67F and Y67L Thermotoga maritima ?
-
?
tRNA uridine55
-
Thermotoga maritima tRNA pseudouridine55
-
?

Synonyms

Synonyms Comment Organism
pseudouridine 55 synthase
-
Thermotoga maritima
psi55S
-
Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
tRNA uridine55 wild-type enzyme Thermotoga maritima