Any feedback?
Literature summary for 5.4.99.28 extracted from
- Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae (2004), J. Biol. Chem., 279, 52998-53006.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharomyces cerevisiae |
| overexpression in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D112A | mutant does not pseudouridine32 in formation in cytoplasmic tRNAGly of the DELTArib2/pus8/DELTApus9 strain | Saccharomyces cerevisiae |
| additional information | Pus9P lacking the 23 N-terminal amino acids is inactive | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tRNA uridine32 | Saccharomyces cerevisiae | Rib2/Pus8p acts specifically on cytoplasmic tRNAs, whereas Pus9p modifies the same position mostly in mitochondrial tRNAs | tRNA pseudouridine32 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | Q12069 | - |
- |
| Saccharomyces cerevisiae | Q12362 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tRNA uridine32 | - |
Saccharomyces cerevisiae | tRNA pseudouridine32 | - |
? | |
| tRNA uridine32 | Rib2/Pus8p acts specifically on cytoplasmic tRNAs, whereas Pus9p modifies the same position mostly in mitochondrial tRNAs | Saccharomyces cerevisiae | tRNA pseudouridine32 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pus9 | - |
Saccharomyces cerevisiae |
| Rib2/Pus8p | - |
Saccharomyces cerevisiae |
| tRNA:Psi32-synthase | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | Rib2/Pus8p acts on cytoplasmic tRNAs, whereas Pus9p modifies the same position in mitochondrial tRNAs. The DELTArib2/pus8/DELTApus9, is viable only in the presence of 15 mg/liter riboflavin in the growth medium | Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
